10 to 20 t g. of each compound are re- 

 quired. IData on melting points, specific 

 rotation, and infrared and near -infrared 

 spectra are included. 



Lambert, M. and A. C. Neish 



1950. Rapid method for estimation of 



glycerol in fermentation solutions . 



Canadian Journal of Research , 28B : 



83-89. 

 Glycerol is oxidized to formaldehyde 

 with periodic acid, the iodate and periodate 

 formed are reduced to iodide with sodium 

 arsenite, and formaldehyde is determined 

 on the oxidation mixture by the chromo- 

 tropic acid color reaction . 



Lea, C. H. and D. N. Rhodes 



1953. Phospholipins. 1. Partition chro- 

 . matography of egg-yolk phospholipins 



^ on cellulose. Biochemical Journal , 54: 



467-469. 

 The authors were unable to confirm the 

 report of Sevan, et al ( Journal of the Chem- 

 ical Society, p. 841, 1951) that ethanolamine 

 and choline -containiQg phospholipids were 

 separable by partition chromatography on 

 paper or cellulose columns. However, ami- 

 no acid contaminants of the phospholipids in 

 ether or chloroform solution were removed 

 by this method. 



Lea, C. H. and D. N. Rhodes 



1954. Phospholipids. 2. Estimation of 

 amino nitrogen in intact phospholipids . 

 Biochemical Journal, 56: 613-618. 



A modification of the ninhydrin method of 

 Moore and Stern ( Journal of Biological Chem - 

 istry, 176 : 367, 1948) for the estimation of 

 phosphatidylethanolamine in the presence of 

 phosphatidylcholine. Preliminary hydrolysis 

 of the phospholipid is unnecessary. 



Lea, C. H. and D. N. Rhodes 



1954. Determination of the iodine value 

 ▲ of phospholipids . Analyst, 79: 304-305. 



The Rosenmund-Kuhnhenn method was 

 found to give results 2 to 10% low on sam- 

 ples of pure methyl esters, and the Yasuda 

 method gave results 4 to 14% low. 



* 



A 



Lea, C. H., D. N. Rhodes, and R. D. StoU 

 1955. Phospholipids. 3. On the chro- 

 matographic separation of glycerophos- 

 pholipids. Biochemical Journal, 60: 

 353-363. 



Phosphatidyl ethanolamine and phospha- 

 tidyl choUne were prepared from egg yolk 

 phospholipids by silicic acid chromatog- 

 raphy using CHCl3-MeOH as eluting solvent. 

 Their lyso -equivalents were pref)ared from 

 venom -treated egg yolk phospholipids in a 

 similar manner. A method and discussion 

 of chromatography of egg yolk phospholipids 

 on silicic acid-impregnated paper is also 

 given. 



Egg lecithin prepared by chromatography 

 on alumina separated into lecithin and lyso- 

 lecithin when rechromatographed on silicic 

 acid. 



Lea, C. H. 



1955. Some observations on the prepara- 

 tion and properties of phosphatidyl eth- 

 anolamine. Proceedings of the Interna - 



A tional Conference on Biochemical Prob - 



lems of Lipids, 2nd Ghent . (Pub. 1956) 

 pp. 81-90. 

 The changes which occur during autooxida- 

 tion of phosphatidyl-ethanolamine are dis- 

 cussed. 



Deoxygenation of the Si02 column before 

 chromatography of phospholipids led to de- 

 creased formation of oxidation products . 



Lees, M. 



1956. Simple procedure for the isolation 

 of brain sulfatides . Federation Proceed- 

 ings, 15: 298. 



Brain white matter is v^xtracted with CHCI3- 

 MeOH and the extract is washed according to 

 Folch, et al ( Federation Proceedings, 13 : 209, 

 1954). The extract is purified by reextraction 

 and the sulfatide is precipitated at -10° . 



Leffler, H. H. 



1959. Estimation of cholesterol in serum. 

 American Journal of Clinical Pathology, 

 ■ 3V. 310-313. 



Iscpropyl alcohol is used for the simul- 

 taneous precipitation of serum proteins and 

 the extraction of total cholesterol. Free 

 cholesterol is precipitated as the digitonide 



42 



