from an aliquot . FeCl3 in 87% H3PO4 is 

 used as color reagent. 



Leupold, F. andH. Buttner 



1953. The hydrolysis of acetals of hi^er 

 fatty aldehydes. Zeitschrift fur physio - 

 logische Chemie, 292: 7-13. Chemical 

 Abstracts, 48:11366F(1954). 

 A study was made of the hydrolysis of di- 

 methyl acetals of palmitaldehyde and stear- 

 aldehyde in AcOH and AcOH-2 N HCl mix- 

 tures. The hydrolysis of the aldehydes was 

 found to be incomplete in 99-100% AcOH, 

 but proceeded readily in 90% AcOH. The 

 aldehydes were determined photometrically 

 at 293 myu . after extracting with cyclohex- 

 ane and drying the extract with Na2S04. 



Leupold, F. and D. Eberhagen 



1959. A simple method for the separation 

 of unsaturated acids from small amounts 

 "A: oflipides. Fette, Seifen, Anstrichmit - 



• tel, 



60: 809-811. 

 53:3738d(1959). 



Chemical Abstracts, 



Polyunsaturated fatty acids were separated 

 by low -temperature urea fractionation. 



Levene, P. A. and I. P. Rolf 



1927. The preparation and purification 



"A" of lecithin. Journal of Biological Chem - 



A istry, 72: 587-590. 



Methods are given for the extraction and 

 purification of lecithin from egg yolk, brain, 

 and liver tissues. The lecithin is precipita- 

 ted from ethanol with CdCl2 and purified by 

 precipitation from chloroform solution with 

 methanol -gaseous ammonia. Analyses of 

 purified products are given. 



Levine, C . and E . Chargaff 



1951. Procedures for the microestima- 

 tion of nitrogenous phosphatide constit- 

 A uents. Journal of Biological Chemistry, 



192 : 465-479. 

 Ethanolamine, serine, and choline, in a- 

 mounts of 5-75^g., were determined as 

 follows: The phosphatides were hydrolyzed, 

 the fatty acids were removed, and the bases 

 were separated by paper chromatography. 

 Ethanolamine and serine were located on 

 the developed chromatogram with ninhydrin 

 and estimated by colorimetry of the eluted 



spots with ninhydrin . Choline spots were 

 converted to choline phosphomolybdate, re- 

 duced to molybdenum blue, and the spots 

 were measured by planimetry. 



Levine, C. and E. Chargaff 



1951. Chromatographic behavior of ana- 

 logues of the nitrogenous lipide constit- 

 uents. Journal of Biological Chemistry, 

 192 : 481-483. 

 Analogues of ethanolamine, serine, and 

 choline were separated by paper chromatog- 

 raphy using several solvent systems, and 

 estimated by the ninhydrin reaction. 



Liberti, A., G. P. Cartoni, and U. Pallotta 

 1958. Vapor -phase chromatography of 

 methyl esters of fatty acids and their 

 . quantitative determination by automatic 



coulometry. AnnaU di Chimica (Rome) 

 • 48: 40-49. Chemical Abstracts : 52: 



9869e (1958). 

 Methyl esters were separated on a sili- 

 cone grease column using glass powder as 

 a support and burned to CO2 at the exit. 

 The CO2 was passed into an ethanolic solu- 

 tion of BaCl2, Ba(0H)2, andH202- The cur- 

 rent used to maintain constant pH of the so- 

 lution, which is a quantitative measurement 

 of the CO2 formed, is recorded graphically. 

 Sensitivity of the method is 0.2 microequiv- 

 alents, and accuracy is 2-5%. 



Lieboff, S. L. 



1928. A colorimetric method for the de- 

 termination of lipoidal phosphorus in 

 A blood. Journal of Biological Chemistry , 



80: 211-214. 

 Lipids are extracted with alcohol -ether, 

 the solvent is evaporated, and organic mat- 

 ter is oxidized with H2SO4 and H2O2. Phos- 

 phorus is precipitated as uranium phosphate, 

 dissolved in trichloracetic acid, converted 

 to uranium ferrocyanide, and the ferrocya- 

 nide is measured colorimetrically. 



Lipmann, F . and L . C . Tuttle 



1945. A specific micromethod for the 

 determination of acyl phosphates. Jour- 

 nal of Biological Chemistry , 159 : 21-28. 

 Acyl phosphates are determined by conver- 

 sion to hydroxamic acid derivatives and 



43 



