Lovem, J. A. 



1956. The lipids of fish. 8. The tri- 

 i^ glycerides and cholesterol esters of 



J[ haddock flesh. Biochemical Journal, 



• 63: 373-380. 



The acetone extract of haddock flesh, con- 

 taining the cholesterol esters and triglycer- 

 ides, was chromatographed on silicic acid 

 with petroleum ether, petroleum ether - 

 benzene, and benzene -ether as eluting sol- 

 vents . Fair separation of the hydrocarbons, 

 cholesterol esters, and triglycerides was 

 achieved. 



Luecke, R. W. and P. B. Pearson 



1944. The determination of free choline 

 in animal tissues. Journal of Biological 

 Chemistry , 155: 507-512. 

 Lecithin is removed by precipitation with 

 acetone. Free choline is then separated by 

 adsorption on Etecalso and elution with 5% 

 NaCl, and assayed with a cholineless strain 

 Neurospora crassa . 



The method gave results in agreement with 

 relneckate precipitation method for the to- 

 tal choline in liver, but chemical methods 

 were not sensitive enough for determination 

 of free choline . 



Lynn, W. S., Jr., L. A. Steele, and E. Staple 

 1956. S^Jaration of 2, 4-dinitrophenyl- 

 hydrazones of aldehydes and ketones 

 -ff by paper chromatography . Analytical 



Chemistry, 28: 132-133. 

 The 2, 4-dinitrophenylhy(lrazones were 

 separated by chromatography on phenoxy- 

 ethanol -impregnated paper using heptane 

 as mobile phase . 



MacGee, J. 



1959. Enzymatic determination of poly - 

 ^ unsaturated fatty acids . Analytical 



• Chemistry , _31: 298-302. 



A method is described for the determina- 

 ticMi of total polyunsaturated fatty acids by 

 oxidation of the potassium salts of the fatty 

 acids with atmospheric oxygen in the pres- 

 ence of lipidoxidase, and spectrophotomet- 

 ric measurement of the conjugated product. 



(Essentially the same as MacGee, et al. 

 Essential Fatty Acids. Proceedings of the 

 International Conference on Biochemical 



Problems of Lipids, 4th Oxford , 

 (Pub. 1958) pp. 21-29.) 



1957, 



McKibbin, J, M. and W. E. Taylor 



1949. The nitrogenous constituents of 

 the tissue lipids. I. The extraction, 

 purification, and hydrolysis of tissue 

 "^ lipids . Journal of Biological Chemistry , 



178 : 17-27. 

 Tissue was extracted with ethanol-ether 

 (3:1), followed by a six-hour continuous ex- 

 traction with chloroform. Non-lipid im- 

 purities were removed by emulsification of 

 a chloroform extract with a 0.25 M MgCl2, 

 freezing to break the emulsion, and removal 

 of the MgCl2 solution. A five-hour Ba(0H)2 

 iiydrolysis was used to free combined cho- 

 line. 



McKibbin, J. M. and W. E. Taylor 



1949. The nitrogenous constituents of 

 the tissue lipids. II. The determina- 

 tion of sphingosine intissue lipid ex- 

 tracts . Journal of Biological Chemis- 

 try, 178: 29-35. 

 The lipid extract was hydrolyzed with 

 saturated Ba(0H)2 and refluxed with HCl, 

 and the liberated sphingosine was extracted 

 with CHClg . The sphingosine was then di- 

 gested for N according to Koch and McMeekin 

 (Journal of the American Chemical Society, 

 46: 2066,' 1924) and determined colorimet- 

 rically . 



The extraction method is claimed to be 

 quantitative and specific in the presence of 

 other lipid bases. The method was used 

 routinely for a range of 6 to 20 umoles of 

 sphingosine, 



McKibbin, J. M. 



1959. Determination of inositol, ethanol- 

 amine, and serine in lipides. Methods 

 of Biochemical Analysis, D. GUck, editor. 

 New York, Interscience Pub. Inc., Vol. 

 7, pp. 111-143. 

 Methods are described for determination 

 of lipid inositol by bioassay with Saccharo- 

 myces carlsbergensis, and colorimetric de- 

 termination of ethanolamine and serine with 

 sodium 1, 2 -naphthoquinone -4 -sulfonate aft- 

 er sealed-tube hydrolysis with 4 N HCl and 

 separation on Permutit. 



45 



