Various other methods for determination 

 of inositol, ethanolamine, and serine are 

 discussed. 



A discussion of factors affecting the de- 

 velopment of maximum color in the Lieber- 

 mann-Burchard reaction. 



MacLachlan, P. L. 



1944. Determination of the iodine niim- 

 ber of whole phospholipid. Journal of 

 Biological Chemistry, 152 : 97-102. 

 When chloroform was used to dissolve 

 phospholipid which had been precipitated 

 with acetone and MgCl2 (Yasuda, Journal 

 of Biological Chemistry , ^: 410, 1931-32) 

 the iodine numbers of the phospholipid 

 were erratic. Reliable iodine numbers 

 were obtained when the chloroform solu- 

 tion of phospholipid was evaporated to dry- 

 ness and the phospholipid redissolved in 

 chloroform. Reliable iodine numbers were 

 also obtained when chloroform -ether (1:1) 

 or moist ether were used as solvents, but 

 neither was as good a solvent for the phos- 

 pholipid as chloroform. It is suggested 

 that the erratic values with the original 

 chloroform solution are due to the forma- 

 tion of a phospholipid-MgCl2 complex which 

 is partially soluble in chloroform . 



Mac Lean, H. 



1914. A simple method for the prepara- 

 ^ tion of lecithin . Journal of Pathology 



A and Bacteriology, 18 : 490-494. 



Lecithin was prepared by a series of 

 precipitations with acetone from an alcohol - 

 water emulsion. 



Ma, T. S. and J. D. McKinley 



1953. Determination of phosphorus in 

 organic compounds: A new mtcropro- 

 cedure. Mikrochimica Acta, 1 : 4-13 

 (In English). Chemical Abstracts, 47 : 

 9858a (1953). 

 A method is described for determination 

 of organic phosphorus using the yellow phos- 

 phovanadomolybdate color in place of molyb- 

 denum blue. 



Machebouef, M . A . and J . L . Delsal 



1942. Colorimetric determination of 

 small quantities of free or esterified 

 _ cholesterol. Bulletin de la Societe de 



chimie biologique, 24: 296-309. Chem - 

 ical Abstracts, 40:103^(1946). 



Mai, S. H. 



1951. Ultramicromethod for separation 

 and determination of fatty acids on nylon 

 "^ thread. Federation Proceedings, 10 : 



• 388. 



A method is described for the Reparation 

 of straight-chain, even numbered, saturated 

 fatty acids from Cg to C^g by chromatog- 

 raphy on nylon thread. The acids are sep- 

 arated into groups, and the individual mem- 

 bers of the groups are separated by use of 

 various solvent mixtures for development. 

 The separated acids are estimated by the 

 monolayer film method. Suitable for deter- 

 mination of 2-6 jug. of each acid in a mix- 

 ture of the acids. 



Makita, T. 



1958. Stability for oxidation of unsaturated 

 fatty acids in their urea adduct crystals. 

 Review of Physical Chemistry of Japan, 



* 28:31-35. (In EngUsh). Chemical Ab - 

 stracts , 53:14546b (1959). 



The urea adduct of unsaturated fatty acids 

 becomes unstable with oxidation in air at 80°, 

 but stabilizes at higher pressures and pro- 

 tects the acid from oxidation by air . 



Mallov, S., J. M. McKibbin, andj. S. Robb 

 1953. The distribution of some of the 

 essential lipides in beef heart muscle 

 and conducting tissue . Journal of Bio- 

 logical Chemistry , 201 : 825-837. 

 Modifications of the anthrone colorimet- 

 ric carbohydrate procedure for use in lipid 

 sugar determination are given. The meth- 

 od was found to be more satisfactory than 

 other reduction and colorimetric procedures . 

 Attempts to apply the selective hydrolysis 

 and reineckate precipitation methods for 

 sphingomyelin to beef heart lipid extracts 

 were unsuccessful. The Brand-Sperry ( Jour- 

 nal of Biological Chemistry, 141: 545, 1941) 

 sugar reduction method and the Bruckner 

 (Zeitschrift fiir physiologische Chemie, 286: 

 163, 1941) orcinol sugar color method were 

 found to be unsatisfactory for beef heart 

 lipid sugar determination. 



46 



