Man, E. B. and E. F. Gildea 



1932. A modification of the Stoddard and 

 Drury tltrimetrlc method for the deter- 

 minatlon of the fatty acids in blood 



* serum . Journal of Biological Chemistry , 



99: 43-60. 

 The alcohol -ether extract of serum is 

 saponified, and the fatty acids are freed with 

 HCl and titrated with NaOH. 



Bloor's extraction procedure gave incom- 

 plete extraction. 



Man, E. B. and J. P. Peters 



1933. Gravimetric determination of 

 serum cholesterol adapted to the Man 

 and Gildea fatty acid method, with a 

 note on the estimation of lipoid 

 phosphorus. Journal of Biological Chem - 

 istry, 101: 685-695. 



A micro modification of the digitonin pre- 

 cipitation method which utilizes the solution 

 left from the Man and Gildea (Journal of Bio- 

 logical Chemistry, 99: 43, 1932) fatty acid 

 determination. Phospholipid is determined 

 by the Fiske-Subbarow method on an aliquot 

 of the same solution . 



Man, E . B . 



1937. A note on the stability and quanti- 



tative determination of phosphatides. 



Journal of Biological Chemistry , 117 : 



183-187. 

 Phosphatide decomposition occurs during 

 the evaporation in air of solvents from solu- 

 tions of phospholipids . Evaporation under 

 N2 at low pressure and temperature avoids 

 the decomposition. 



Man, E. B. and E. F, Gildea 



1937. Notes on extraction and saponifica- 

 tion of lipids from blood and blood 

 " serum . Jouraal of Biological Chemistry , 



122: 77-88. 

 No significant difference was found in the 

 use of NaOH or KOH for saponification, con- 

 trary to earlier findings . 



Refluxing of blood with alcohol -ether for 

 1 hour gave greater yield of lipid phosphorus 

 than heating for 1 to 5 minutes, and higher 

 yields were also obtained when the reflux- 

 ing was carried out in an inert atmosphere . 



Mangold, H. K., B. G. Lamp, and H. Schlenk 

 1955. Indicators for the p^-^er chroma- 

 ^ tography of lipids . Journal of the Amer - 



lean Chemical Society, 77: 6070-6072. 

 Indicators are described for the detection 

 of various lipids on paper chromatograms . 



Marcali, K. andW. Rieman, in 



1946. Microdetermination of the sapon- 

 ification number of fats and oils. In- 

 dustrial and Engineering Chemistry, 

 Analytical Edition , 18: 144-145. 

 A description of methods for determina- 

 tion of the saponification number of fats and 

 oils using samples of about 500, 50 and 15 

 mg. 



Marenzi, A. D. andC. E. Cardini 



1943 . Colorimetric micromethod for 

 determining total and unsaturated fat 

 acids of blood. Revista de la Sociedad 

 • Argentina de Biologia , 19: 118-130. 



Chemical Abstracts, 38: 563^ (1944). 

 Blood lipids are extracted and the fatty 

 acids are converted to Pb soaps . The Pb is 

 then precipitated from the soaps as PbCrO^ 

 and the Cr is determined. 



Marenzi, A. D. andC. E. Cardini 



1943. The colorimetric determination of 

 choline • Journal of Biological Chemistry , 

 147 : 363-370. 



Seattle's method ( Biochemical Journal, 30 : 

 1554, 1936) was modified as follows: 

 1) 60% acetone in water was used to dissolve 

 the reineckate, eliminating the problems of 

 evaporation caused by use of acetone alone . 

 Lower concentrations of acetone did not dis- 

 solve the salt completely, or else gave rise 

 to a precipitate on standing. 2) The choline 

 reineckate was precipitated by a 20 minute 

 immersion in ice water, as precipitation 

 was found to be complete under those condi- 

 tions. 



A new method for the determination of 

 choline was proposed, based on the precip- 

 itation of choline as the reineckate and col- 

 orimetric determination of the chromium 

 in the precipitate, which is sensitive to 15 

 yuLg. of choline. See also: Lovern, Chemis- 

 try and Industry, 707, 1950. 



47 



