Marenzi, A. D. andC. E. Cardini 



1943. On the determination of the phos- 

 pholipids in blood. Journal of Biolog- 

 ical Chemistry, 147 : 371-378 . 

 Total lipid phosphorus was determined 

 by the Fiske-Subbarow method, sphingo- 

 myelin by determination of the phosphorus 

 content of its reineckate, and choline by 

 determination of the chromium content of 

 its reineckate. Satisfactory results were 

 not obtained by colorimetric measurement 

 of sphingomyelin reineckate. 



Marinetti, G. V. andE. Stotz 



1955. Paper chromatographic separation 

 •^ of phospholipids. Journal of the Amer- 

 A ican Chemical Society, 77 : 6668-6670. 



Phosphatidylethanolamine and acetal phos- 

 pholipid were acylated with acetic anhydride, 

 benzoyl chloride, or 2, 4-dinitroflouroben- 

 zene and separated by chromatography on 

 paper with solvent mixtures containing 

 lutidine, acetic acid, and methanol or oc- 

 tanol . 



Marinetti, G. V. and E. Stotz 



1956. Chromatography of phosphatides 

 on silicic acid impregnated paper. 

 Biochimica et Biophysica Acta, 21: 

 168-170. 



Phosphatides were separated on silicic 

 acid-impregnated paper using diisobutyl 

 ketone -acetic acid-water (40:30:7) or 

 n-butyl ether -acetic acid-chloroform - 

 water (40:35:6:5) as solvents. 



Marinetti, G. V., J. Erbland, and J. Kochen 



1957. Quantitative chromatography of 

 ■^ phosphatides . Federation Proceedings, 

 A 16: 837-844. 



Methods are described for the separation 

 of phosphatides on silicic acid -impregnated 

 paper and silicic acid columns. EWisobutyl 

 ketone -acetic acid-water and diisobutyl 

 ketone -n-butyl ether -acetic acid-water, in 

 various ratios, are used as developing sol- 

 vents . 



Washing the silicic acid with methanol- 

 chloroform before use gave more repro- 

 ducible results than activation by heating, 

 (cf . Kay and Trueblood, Analytical Chem- 

 istry, 26: 1566, 1954). 



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Marinetti, G. V., J. Erbland, andE. Stotz 



1959. The quantitative analysis of plas- 



malogens by paper chromatography. 



Biochimica et Biophysica Acta, 31 : 



251-252. 



Lecithin and phosphatidylethanolamine 

 are isolated by silicic acid chromatography 

 and hydrolyzed with acetic acid. The re- 

 sulting lysophosphatides are separated by 

 paper chromatography. The plasmalogen 

 content of the original lipid is calculated 

 from the amount of lipid phosphorus in the 

 lysolecithin or lysophosphatidylethanol- 

 amine. 



Marinetti, G. V., M. Albrecht, T. Ford, and 

 E. Stotz 



1959. Analysis of human plasma phos- 

 phatldes by paper chromatography. 

 Biochimica et Biophysica Acta, 36: 

 ^ 4-13. 



Plasma lipids were extracted and chro- 

 matographed on silicic acid-impregnated 

 paper using diisobutyl ketone-acetic acid- 

 water (40:25:5) as solvent. The phospha- 

 tide spots were extracted with methanolic 

 HCl and digested with perchloric acid and 

 phosphorus was determined by spectro- 

 photometric measurement of the phospho- 

 molybdate . 



Marquardt, P. andG. Vogg 



1952. A sensitive assay of choline and 

 acetylcholine by means of sodium 

 tetraphenylboron . Zeitschrift fur 

 physiologische Chemie, 291: 143-147. 

 Chemical Abstracts, 48: 12855e (1954). 

 Choline or acetylcholine is precipitated 

 quantitatively by tetraphenylboron in acid 

 solution. Data on infrared absorption spec- 

 tra and solubilities of the complexes are 

 given. 



Mata, M. 



1948. Simplified determination of blood 

 lipides . Re vista Farmaceutica de Cuba , 

 ■ 26: 29-31. Chemical Abstracts , 43: 



38711 (1949). 

 The sample of blood is dried on filter pa- 

 per. The lipids are extracted with Et20- 

 EtOH(5:l), dried, and weighed. Choles- 

 terol is extracted from the residue with 



48 



