CHCI3 and determined by the Liebermann- 

 Burchard reaction . 



Matthews, F. W., G. G. Warren, and 

 J. H. Michell 



1950. Derivatives of fatty acids. Ana- 



# lytical Chemistry, 22: 514-519. 



X-ray diffraction powder patterns of sil- 

 ver salts, amides, and anilides of fatty 

 acids were studied and found to be suitable 

 for fatty acid identification . Methods for 

 preparation of the derivatives and data on 

 their X-ray diffraction are given. 



Mead, J. F. 



1957. The metabolism of the essential 

 fatty acids. VI. Distribution of un- 



. saturated fatty acids in rats on fat -free 



supplemented diets . Journal of Biolog- 

 ical Chemistry, 227 : 1025-1034. 

 Unsaturated fatty acids were chromato- 

 graphed on siliconized Celite, hydrogenated, 

 and rechromatographed. Acids with differ- 

 ing degrees of unsaturation were well sepa- 

 rated. 



Meredith, P. andH. G. Sammons 



1952. Horizontal paper chromatography. 



if Analyst, 77: 416-418- 



A method for horizontal paper chroma- 

 tography is described. The method was 

 used to separate the water-soluble constit- 

 uents of a methanolic-HCl hydrolysate of 

 nerve lipids . 



Meredith, P. andH. G. Sammons 



1959. A reaction between meso-inositol 

 and uranyl acetate. Analyst, 83 : 686. 

 Inositol can be detected on paper chroma - 

 tograms by its flourescence when sprayed 

 with uranyl acetate. 



Michaels, G. D., G. Fukayama, H. P. Chin, 

 and P. Wheeler 



1958. Technics for separation of plasma 

 cholesterol esters for determination of 



-if iodine value, and of cholesterol. Pro- 



■ ceedings of the Society for Experimental 



Biology and Medicine, 98: 826-829. 

 Cholesterol esters are separated from 

 blood lipids by chromatography on silicic 

 acid. Iodine number is determined by a 



colorimetric modification of Yasuda's 

 method. Cholesterol is determined by a 

 modification of the Schoenheimer-Sperry 

 method using an orcinol -ferric chloride 

 color reagent. 



Michaels, G. D. 



1958 . A note on increasing the accuracy 

 of the micro alkaline isomerization 

 _ technic for determination of unsaturated 



fatty acids . American Journal of Clin- 

 ical Nutrition , 6: 593-594. 

 Absorption due to reagent blank in the 

 method of Herb and Riemenschneider (Ana- 

 lytical Chemistry, 25: 953, 1953) is reduced 

 by acidification of the isomerized fatty acids, 

 extraction of the fatty acids with petroleum 

 ether, evaporation of the extract to dryness, 

 and dissolving the fatty acid residue in 

 methanol for spectrophotometric reading. 



Michaels, G. D., P. Wheeler, G. Fukayama, 

 and H . P . Chin 



1958. Column chromatographic fractiona- 

 ^ tion of plasma lipids; American Jour- 



• nal of Clinical Nutrition , 6: 604-605. 

 A method is described for separation of 



cholesterol esters, free cholesterol, and 

 mono-, di-, and triglycerides from blood 

 plasma lipids by chromatography on silicic 

 acid using petroleum ether and diethyl ether 

 in varying concentrations as eluents. Re- 

 covery of added glycerides is quantitative. 



Michaels, G. D., P. Wheeler, G. Fukayama, 

 and L. W^ Kinsell 



1959. Plasma cholesterol fatty acids in 

 human subjects as determined by alka- 

 line isomerization and by gas chroma- 



# tography . Annals of the New York 

 Academy of Sciences, 72 (art. 13): 

 633-640. 



Cholesterol was determined by a modifica- 

 tion of the Schoenheimer and Sperry method 

 using orcinol for color development. 



A procedure is described for determina- 

 tion of unsaturated fatty acids in amounts 

 of approximately 0.3 mg. by a modification 

 (Michaels, American Journal of Clinical 

 Nutrition, 6: 593, 1958) of the spectrophoto- 

 metric method of Herb and Riemenschneider 

 (Analytical Chemistry, 25: 953, 1953). 



49 



