The microisomerization method of Hoi- 

 man (in Methods of Biochemical Analysis, 

 D. Click, Editor, 1957, vol. 4, p. 99) 

 was found to give errors as hig^ as 50%, 

 presumably due to the high blank. 



Michalec, C. 



1957. The nature of cholesteryl esters in 

 the higher fatty acids in human blood 



j^ serum. Essential fatty acids. Proceed - 



^ ings of the International Conference on 



* Biochemical Problems of Lipids, 4th 

 ■ Oxford , (Pub. 1958) pp. 105-110. 



Chemical Abstracts, 53: 17278h (1959). 

 Cholesterol and cholesteryl esters were 

 separated from blood lipid extracts by chro- 

 matography on paraffin oil -impregnated 

 paper using aaAcOH-CHCU -paraffin oil 

 (65:25:10) mobile phase. Higher fatty acids 

 were separated by using 93-95% AcOH as 

 mobile phase. "Critical pairs" were sepa- 

 rated by KMn04 oxidation of the unsaturated 

 fatty acids prior to chromatography. 



Michalec, C . 



1958. Two-dimensional paper chroma- 

 . tography of hi^er fatty acids . Bio- 

 _ chimica et Biophysica Acta , 28: 212- 



• 213. ~~ 

 Fatty acids were separated by two- 

 dimensional chromatography on paraffin 

 oil -impregnated paper. The first chroma - 

 togram was run at 20° using 93% acetic 

 acid, and the second chromatogram was 

 run at -8° using formic acid-acetic acid- 

 water as solvent. 



Molines, J. and P. Desnuelle 



1948. Colorimetric determination of 



phosphorus in oils and lecithins. 



Bulletin mensuel d'information ITERG , 



No. 2 : pp 1 -3 . Chemical Abstracts', 



42:4493d (1948). 

 The sample is digested with HNO3 and 

 H2SO4 and phosphorus is determined col- 

 orimetrically, using metol as reducing 

 agent for molybdic acid. As little as 0.01 

 mg. of phosphorus can be determined by 

 this method. 



Monasterio, G. and G. Gigli 



1947. A new method for the study of the 



lipides of feces. Rassegna de fisio- 

 patologica clinica e terapeutica (Pisa), 

 29: No. 11/12, 33 pp. Chemical Ab- 

 stracts, 43:2658e (1949). 

 Lipids are extracted from feces with ace- 

 tone and ether. Directions are given for 

 the determination of the individual lipids . 



Moretti, J. and J. Polonovski 



1954 . Chromatographic separation of 

 bromine derivatives of stearic acid. 

 ■^ Bulletin. Societe chimique de France, 



• pp. 935-936. Chemical Abstracts, 48: 

 13544a (1954). 



Bromo derivatives of stearic acid are 

 separated by chromatography on paper or 

 a cellulose or Cellte column, and the com- 

 pounds are eluted according to their solu- 

 bilities in petroleum ether, ethyl ether, 

 dichloroethylene and trichloroethylene . 



Morgan, D. M. and K.J. Kingsbury 



1959. A modified hydroxamic acid meth- 

 _ od for determining total esterified fatty 



acids in plasma . Analyst, 84:409-414. 

 The esterified fatty acids in plasma were 

 converted to their hydroxamates and the col- 

 ored complex formed with ferric chloride 

 was measured at 515 m^. Variation + 0.85%. 

 Recoveries from glyceryl trioleate = 99.7%. 



Moyle, v., E. Baldwin, andR. Scarisbrick 

 1949. Separation and estimation of sat- 

 urated C2-Cg fatty acids by buffered 

 ^ partition columns. Biochemical Jour- 



• nal, 43 : 308-317. 



The fatty acids are separated on a heavily 

 buffered silica gel column with butanol- 

 water mixtures as elutlng solvents. The 

 separated acids are estimated by titration. 

 Effects of variables and applications of the 

 method are discussed. 



Mukerjee, H. 



1959. Paper chromatography of volatile 

 if fatty acids. Analytical Chemistry , 31: 



• 1284. ~ 

 MeOH-NH3 and MeOH-acetone-NH3 sol- 

 vent mixtures are used to separate the am- 

 monium salts of formic, acetic, propionic, 

 and butyric acids by paper chromatography . 



50 



