and the combined water-soluble choline 

 in the effluent is hydrolyzed and deter- 

 mined separately as the periodide . 



Duffie, M. J. and J. L. Guravich 



1959. A comparison of the methods of 



Bloor and Schoenheimer-Sperry in the 



estimation of cholesterol in serum. 



American Journal of Clinical Pathology, 



32: 92-96. 

 Serum cholesterol determinations by the 

 Bloor (Journal of Biological Chemistry, 24: 

 227, 1916) and the Sperry and Webb modifica- 

 tion (Journal of Biological Chemistry, 187: 

 97, 1950) of the Schoenheimer-Sperry meth- 

 od were done in parallel. The Bloor method 

 yielded values which were 1.044 times as 

 large as comparable values by the S-S meth- 

 od. The Bloor method appeared to be as 

 accurate, and was faster than the S-S meth- 

 od. 



Dutton, H.J. and C . L . Reinbold 



1948. Adsorption analysis of lipides. 

 in. Synthetic mixtures of ethyl stear- 

 ic ate, oleate, linoleate, and linolenate . 

 ^ Journal of the American Oil Chemists 

 • Society, 25: 120-124. Chemical Ab- 

 stracts, 42:3973f (1948). 

 Details are given of a study of adsorp- 

 tion analysis of binary mixtures of ethyl 

 stearate, oleate, linoleate, and linolenate 

 on alumina columns . 



Edman, P. V. 



1942. A micromethod for the estimation 

 of cerebrosides in nerve tissue. Jour- 

 nal of Biological Chemistry , 143: 219- 

 221. 

 The carbazole reaction of Dische (Mikro- 

 chemie, 8: 4, 1930) is used for the quantita- 

 tive estimation of 0.2 to 0.6 mg. of cerebro- 

 sides by colorimetry. After hydrolysis of 

 the cerebrosides, the fatty acids are remov- 

 ed by virtue of their Insolubility in water, 

 and glycerol is extracted into ethyl acetate. 

 Noll's ( Zeltschrift fur physiologische 

 Chemie, 27: 370, 1899), Kimmelsteil's 

 ( Blochemische Zeltschrift, 212: 359, 1929), 

 and Kirk's (Journal of Biological Chemistry , 

 123fc 613, 1938) methods for estimation of 

 galactose set free from the cerebrosides by 



hydrolysis are criticized for their lack of 

 specificity In reduction methods. 



Edman, P. V. andS. E. G.Aqulst 



1945. A micromethod for the estimation 

 of phosphatidyl ethanolamlne in nerve 

 ▲ tissue. Acta physlologlca Scandlnavica , 



20: 144-149. 

 Phosphatidyl ethanolamlne is estimated as 

 ethanolamlne by hydrolysis of an alcoholic 

 tissue extract, distillation of the ethanol- 

 amlne, and colorlmetric measurement of 

 the blue color developed with sodium hypo- 

 chlorite and phenol. 



Edsgaard, J. 



1948. On the determination of the phos- 

 phatide content of serum. Acta physlo - 

 loglca Scandlnavica , 16: 171-178. 

 An investigation of methods for the deter- 

 mination of serum phospholipid. It was 

 found that absolute ethanol alone is as effec- 

 tive for the extraction of serum phospho- 

 lipids as alcohol-ether (3:1) or alcohol- 

 acetone (1:1). Temperature and time of ex- 

 traction are not critical as long as 1:20 

 ratio of serum to solvent is maintained. 



Edsgaard, J. 



1948. On the colorlmetric determination 

 of phosphorus with "amidol" . Acta 

 A physlologlca Scandlnavica, 16: 179- 



182. 

 A discussion of the effects of variables in 

 the determination of phosphorus with "ami- 

 dol" (diaminophend hydrochloride) and molyb- 

 date. Acidity and temperature of color de- 

 velopment were found to be Important, but 

 age of the reagents had little effect. 



Ellis, G. and L. A. Maynard 



1937. TTie determination of phospholipids 

 in bovine blood. Journal of Biological 



^ Chemistry, 118: 701-709. 



The values for Bloor's oxidative technique 

 (Journal of Biological Chemistry, 82 : 273, 

 1929) were found to be variable and also 50% 

 or more below values obtained by the lipid 

 phosphorus method (method not stated). The 

 values for phospholipid found in the final 

 moist ether (or chloroform) solution after 

 the purification steps of Bloor, however. 



18 



