309-312. Chemical Abstracts, 46: 

 11036b (1952). 

 Phospholipid is precipitated with a meas- 

 ured amount of MgCl2 "61120 in the presence 

 of NH4 salt and NH3, and excess Mg is ti- 

 trated with Complexon III. 



Delsal, J. L. 



1944. New procedure for extraction of 

 serum lipides with methylal. Applica- 

 tion to microdetermination of total cho- 

 . lesterol, phosphoaminolipides and pro- 



teins. Bulletin de la Societe de chimie 

 biologique , 26: 99-105. Chemical Ab- 

 stracts, 39:356CP (1945). 



Extraction of serum cholesterol and lipids 

 with methylal was found to be complete . 



Delsal, J. L. 



1946. Colorimetric microdetermination 

 of free cholesterol . Bulletin de la So- 



■ ciete de chimie biologique , 28: 441-445. 

 Chemical Abstracts, 41:4188a (1947). 



A method is described for the determina- 

 tion of free cholesterol by precipitation with 

 natigen and measurement by the Liebermann- 

 Burchard reaction. 



Delsal, J. L. 



1950. Extraction of cholesterol from 



blood serum by chloroform in the pres- 

 ence of anhydrous sodium sulfate or 



■^ plaster of paris. Compte rendu hebdo- 



■ madaire des seances et m^moires de la 

 Socigtfe de biologie , 144 : 67-69. Chem - 

 ical Abstracts, 44:100241(1950). 



Cholesterol is not completely extracted 

 from serum which is dehydrated with an- 

 hydrous sodium sulfate unless the serum is 

 first hydrolyzed with NaOH. 



Delsal, J. L. landMme. DeMont-Argant 



1950. Accord between the colorimetric 



and gravimetric (by natigin or digitonin) 

 determinations of cholesterol in normal 

 and pathological human serum. An a- 

 nomaly observed with certain lipoid 

 nephrosis serums. Compte rendu heb - 

 domadaire des seances et mfemoires de 

 la Societe de biologie , 144: 87-89. 

 Chemical Abstracts, 44:10024g (1950). 

 Cholesterol values obtained by colorimet- 



ric microdetermination after saponification 

 usually agree with those obtained by gravi- 

 metric determination. 



Delsal, J. L. 



1954. Fractionation of the lipides of 

 blood serum by organic solvents. 

 . Bulletin de la Societe de chimie biolog- 



ique, 36: 1329-1334. Chemical Ab- 

 stracts, 49:7628a (1955). 

 A methylal -methanol (4:1) mixture is used 

 to precipitate protein and extract the lipids 

 from a serum sample. Petroleum ether and 

 water are added to the extract to cause the 

 extract to separate into two phases. Ster- 

 ides and triglycerides concentrate in the up- 

 per phase, and phosphoaminolipides in the 

 lower . 



Devis, R. 



1944. Acidimetric determination of total 

 fat acids in very small quantities of bio- 

 logical fluids and tissues. Bulletin de la 

 % Societe de chimie biologique , 26 : 232- 



238. Chemical Abstracts, 40:1190^ 

 (1946). 

 Lipids are hydrolyzed and acidified to free 

 the fatty acids. The acids are extracted 

 with benzene and titrated with KOH . 



Dieckert, J. W. andR. Reiser 



1956. A paper chromatographic proce- 

 dure for separating 1-mono-, 1,3-di-, 

 ■^ and triglycerides, cholesterol, and cho- 



# lesterol esters. Journal of the American 



■ Oil Chemists Society, 33: 123-126. 



Chemical Abstracts, 50:7012g (1956). 

 1-mono, 1,3-di-, and triglycerides, cho- 

 lesterol, and cholesterol esters were sepa- 

 rated by chromatography on silicic acid-im- 

 pregnated glass fiber paper using ethyl ether- 

 isooctane mixtures as solvents. Spots on the 

 developed chromatogram were detected by 

 spraying with 50% aqueous H2SO. and gen- 

 tle heating to detect cholesterol-containing 

 lipids, followed by stronger heating to de- 

 tect the glycerides. Degree of unsaturation 

 of the glyceride fatty acids had no detect- 

 able effect on the R, values of the triglycer- 

 ides. 



The glass paper was specially washed in 

 MeOH and ethyl ether to remove impurities . 



16 



