Coleman, CM. and G. Middlebrook 



1957. Interface enrichment of methylene 

 ^ blue by fatty acids with micro -analytic 



applications. Science , 126: 163. 

 A heptane solution of fatty acids is enrich- 

 ed by methylene blue when in contact with an 

 aqueous solution of the dye. Spectrophoto- 

 metric measurement of the enriched heptane 

 solution is used for determination of the a- 

 mount of fatty acids present. 



Collins, F. D. andL. W. Wheeldon 



1958. Studies on phospholipids. 4. De- 

 termination of ethanolamine and se- 

 rine. Biochemical Journal , 70 : 46-49. 



After extraction and dinitrophenylation, 

 the lipids are hydrolyzed in ethanolic HCl . 

 The hydrolysate is dried and dissolved in a 

 solution of dimethylformamide-ethanol-n- 

 butylamine, and tetramethylammonium hy- 

 droxide is added. The ethanolamine and 

 serine values are then determined by differ- 

 ential color measurement at 393 mu. and 



500 m 



y>, 



Collins, F. D. 



1959. Studies on phospholipids. 5. The 

 separation of dinitrophenylated and 

 methylated phospholipids by counter - 

 current distribution. Biochemical 

 Journal , 72: 281-287. 

 A method is described for the separa- 

 tion of phospholipids from sheep and rat 

 brain lipids by counter-current distribu- 

 tion of their dinitrophenyl and methyl de- 

 rivatives in several solvent systems. 



Colman, D. M. and A. F. McPhee 



1956. An improved method for deter- 

 mination of total serum cholesterol. 

 American Journal of Clinical Pathol- 

 ogy , 26: 181-186. 

 Direct saponification of the serum sample, 

 extraction of the cholesterol, precipitation 

 with digitonide, and color development with 

 a modified Liebermann-Burchard reagent 

 are all carried out in the same tube. Accu- 

 racy and precision are good, 



Corcoran, G. B. 



i^ 1956. Chromatographic separation and 



# determination of straight -chain satu- 



rated monocarboxylic acids C^ through 

 Cjo ^i^d dicarboxylic acids C, , through 



^16- 

 The acids were separated by chromato- 

 graphy on a glycine -buffered column of si- 

 licic acid using a 1-butanol -chloroform mo- 

 bile phase. Accuracy is+ 1%. 



Cormier, M. and P. Jouan 



1957. Separation of lipides by chroma- 

 tography on paper impregnated with si- 



_. licic acid. Bulletin de la Societe de 



* chimie biologique, 39: 1321-1327. 

 Chemical Abstracts, 53: 11491a (1959). 



A method for the separation of lipids by 

 chromatography on silicic acid-impreg- 

 nated filter paper . 



Cormier, M. and P. Jouan 



1958. Separation of the total lipides of 

 "^ blood serum by chromatography on 



# paper . Bulletin de la Society de chimie 

 A biologique, 40: 171-176. Chemical Ab - 

 ■ stracts, 53:18146b (1959). 



A method is described for the separation 

 of serum lipids by chromatography on silic- 

 ic acid impregnated paper. The phospho- 

 lipids are first separated by chromatography 

 with a Et20-petroleum ether -Me2CO(2: 100:50) 

 solvent mixture, and the glycerides, ster- 

 oids, and cholesterol are then separated by 

 using Et20-petroleum ether -heptane (4:50:50). 



Cormier, M., P. Jouan, and L. Girre 



1959. Separation of lipides by chroma- 

 tography on paper impregnated with si- 



. licic acid. II. Possibilities and Umita- 



2. tions of the method. Bulletin de la So- 



ciete de chimie biologique , 41: 1037- 

 " 1046. Chemical Abstracts, 54:4736e 



(1960). 

 Lipids were separated into steroid, tri- 

 glyceride, and mono- and diglyceride groups 

 by chromatography on Si02~ impregnated 

 paper using an ether -petroleum ether -hep- 

 tane (8:50:50) solvent mixture. The appli- 

 cations of the method are discussed. 



Comer, M. 



1959. Rapid microdetermination of organ- 

 A ically bound halogens, arsenic, phos- 



phorus, and boron. Analyst, 84: 41-46. 



14 



