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Ethanolamine and choline in the phospha- 

 tide fraction were separated as ethanol- 

 amine 3, 5-diiodosalicylate and choline 

 chloride -6 HgCl2 double salt. 



Chargaff, E., C. Levine, andC. Green 



1948. Technique for the demonstration 

 by chromatography of nitrogenous 

 lipide constituents, sulfur-containing 

 amino acids, and reducing sugars. 

 Journal of Biological Chemistry , 175: 

 67-71. 

 HCl hydrolysis and paper chromatography 

 were used to separate components. Tested 

 for choline with phosphomolybdic acid, ethan- 

 olamine and serine with ninhydrin, and re- 

 ducing sugars with m-phenylenediamine di- 

 hydrochloride . 



Chen, P. S., Jr., T. Y. Toribara, and 

 H. Warner 



1956. Micro determination of phosphor- 

 us. Analytical Chemistry , 28 : 1756- 

 1758. 

 A modification of the method of Ammon 

 and Hinsberg ( Zeitschrift fiir physiolog- 

 ische Chemie , 239 : 207, 1936) for use in 

 determining as little as . 15 ng . of phos - 

 phorus in blood and urine. Ascorbic acid 

 is used to reduce phosphomolybdate . Ef- 

 fects of variables are given. Results are 

 identical with Fiske-Subbarow method. 

 The method is suitable for lipid phosphor- 

 us determination. 



Chiamori, N. and R.J. Henry 



1959. Study of the ferric chloride meth- 

 od for determination of total cholester- 

 ol and cholesterol esters. American 

 Journal of Clinical Pathology , 31: 305- 

 309. ~ 



FeCl3 -acetic acid reagent is used to pre- 

 cipitate serum proteins, and the cholesterol 

 color is developed with H2SO4. Bromine in- 

 terference can be avoided by removing it 

 with an ion -exchange resin. 



Traces of digitonide interfered with the 

 determination of cholesterol by the method 

 of Pearson ( Analytical Chemistry, 25: 813, 

 1953), using p-toluenesulfonic acid. Excess 

 silver iodate will interfere with the color 

 reaction (Rice and Lukasiewicz, Clinical 



Chemistry, 3: 160, 1957). 



Christl, H. 



1953. Determination of acetal phospha- 

 tide in tissues. Zeitschrift fiir physio - 

 ▲ logische Chemie, 293: 83-88. Chem- 



ical Abstracts, 49: 13346d (1955). 

 A modification of the method of Feulgen 

 ( Zeitschrift fiir physiologische Chemie , 287 : 

 90, 1951) for use in the determination of 

 tissue acetal phosphatides. 



Clayton, M. M., P. A. Adams, G. B. Mahoney, 

 S. W. Randall, and E. T. Schwartz 



1959. Micro methods for the determina- 

 _ tion of chylomicron counts, fatty esters, 



lipid phosphorus, and cholesterol in 

 blood serum . Clinical Chemistry , 5: 

 426-444. 

 Procedures for determination of lipid 

 components on 75yul . samples of blood 

 serum are given. After alcohol -ether ex- 

 traction, the fatty esters are determined 

 by colorimetric measurement of the ferric 

 hydroxamate complex color developed with 

 acidified ferric perchlorate (Hill, Industri- 

 al and Engineering Chemistry, Analytical 

 Edition , 18: 317, 1946, Ibid. 19: 932, 1947, 

 and Bauer and Hirsch, Archives of Biochem - 

 istry, 20: 242, 1949). 



Phospholipids are oxidized with H2SO4 

 and H2O2 and determined by the Fiske- 

 Subbarow colorimetric method. Cholester- 

 ol esters are hydrolyzed with benzyltri- 

 methylammonium hydroxide and total cho- 

 lesterol is determined by color development 

 with H2S04-acetic anhydride. 



Cole, P. G., G. H. Lathe, and C . R. J. Ruthven 

 1953. The application of counter -current 

 methods to the fractionation of lipid ma- 

 terial from brain. Biochemical Journal, 

 54: 449-457. 

 A water-methanol-carbon tetrachloride 

 solvent system is described for use in coun- 

 ter-current fractionation of brain lipids, and 

 the behavior of the lecithin, cephalin, and 

 sphingomyelin-cerebroside fractions is de- 

 scribed. Variations of the solvent system 

 and its uses are discussed. 



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