* 



action on leech muscle of the acetylcho- 

 line formed. 



Cardini, C. E. andM. E. Serantes 



1943. Methods of extracting sphingo- 

 myelin. Anales de farmacia y bio- 

 quimica. Buenos Aires, 14: 123-132. 

 Chemical Abstracts, 38:4'6307 (1944). 

 Initial extraction of material by Bloor's 

 method ( Tournal of Biological Chemistry , 82: 

 273, 1929), followed by reextraction of the 

 tissue by the method of Thannhauser (Jour- 

 nal of Biological Chemistry, 129: 709, 1939) 

 is recommended. 



Carlson, L. A. and L. B. Wadstrom 



1958. A colorimetric method of deter- 

 mining unesterified fatty acids in 



^ plasma . Scandinavian Journal of Clin- 



ical and Laboratory Investigation, 10: 

 407-414. ~ 



The lipids are extracted with chloroform - 

 methanol (2: 1) . Phospholipids are separated 

 on silicic acid, and fatty acids on Amberlite 

 IRA-400 resin. The methylated fatty acids 

 are determined colorimetrically as the hy- 

 droxyamic acids. Two milliliters of plasma 

 may be used for determination of unesteri- 

 fied fatty acids, glycerides, phospholipids, 

 and cholesterol. The method is sensitive 

 to amounts of fatty acid from 0.5 to 4.0 

 ^equivalents. With 2 . ^ equivalent, re- 

 covery was 97.6% and error + 2.5%. 



Carlson, L . A . and L . B . Wadstr'dm 



1959. Determination of glycerides in 

 ♦ blood serum . Clinica Chimica Acta, 



4: 197-205. 

 Glycerides are separated from phospho- 

 lipids by silicic acid chromatography and 

 saponified. The glycerol is then determin- 

 ed by periodic acid oxidation and estimation 

 of the formaldehyde formed. 



Carpenter, K. J., A. Gostis, and D. M. Heg- 

 sted 



1957. Estimation of total cholesterol in 

 serum by a micro method. Clinical 

 Chemistry, 3: 233-238. 

 An adaptation of the method of Albers and 

 Lowry (Analytica l Chemistry . 27: 1829, 1955) 

 for use in determination of blood serum 



cholesterol. H2SO4 is added to a 



1, 1, 2-trichloroethane-acetic anhydride 

 solution of cholesterol, and the floures- 

 cence which develops is measured . 



Cavanaugh, D. J. and D. Click 



1952. Determination of cholesterol in 

 microgram quantities of tissue . Ana- 

 lytical Chemistry, 24: 1839- 1841 ] 

 A microadaptation of the Sperry-Webb 

 method (Journal of Biological Chemistry, 187 : 

 97, 1950), for use in analysis of cholester- 

 ol in microtome sections of tissue. Error 

 of the method ranges from 1.5 to 4.5% de- 

 pending on sample size . 



Chapman, R. A. andK. MacKay 



1949. The estimation of peroxides in 

 fats and oils by the ferric thiocyanate 

 ^ method. Journal of the American Oil 



Chemists Society, 26: 360-363. Chem- 

 ical Abstracts , 43:68"40h (1950). 

 It was found, in agreement with the find- 

 ings of Lea (Journal of the Society of Chem - 

 ical Industry, 64: 106, 1945), that atmos- 

 pheric oxygen interferes with the determina- 

 tion of peroxide by the ferric thiocyanate 

 method. Exclusion of air and use of nitro- 

 gen atmosphere markedly reduced the per- 

 oxide values determined by the ferric thio- 

 cyanate method. 



Charlampous, F. C. and P. Abrahams 



1957. Biochemical studies on inositol. 

 I. Isolation of myo- inositol from yeast 

 and its quantitative enzymatic estima- 

 tion. Journal of Biological Chemistry , 

 225: 575-583. 

 Methods for the extraction, purification, 

 and crystallization of myo - inositol 

 from yeast cells are described. A method 

 is described for the colorimetric estima- 

 tion of inositol using the reduction of 



2, 6-dichlorophenolindophenol by inositol 

 dehydrogenase in the presence of inositol. 



Chargaff, E. 

 1 1942. A study of Lipoproteins . Journal 

 * of Biological Chemistry, 142: 291-504. 



Ether extraction and dialysis were used 

 to remove "lipovitellin" from egg yolk, and 

 from it removed the phosphopha tides. 



12 



