thin into CdS by exposure to H2S . 



Burmaster, C. F. 



1946. Microdetermination of OtandQ 

 glycerophosphates. Journal of Biolog - 

 ical Chemistry , 164 : 233-240. 

 Results of colorimetric measurement of 

 the orthophosphate produced by the reac- 

 tion of periodate on a glycerophosphate fol- 

 lowed by acid hydrolysis agree with those 

 obtained by titration of the reduced period- 

 ate. Microcolorimetric methods suitable 

 for the analysis of sodium glycerophosphate 

 and phosphorus compounds in phospholipid 

 hydrolysates, for inorganic phosphorus, cx 

 phosphorus, (cX + "S" ) phosphorus, and to- 

 tal phosphorus are described. 



ing the developed chromatogram with nin- 

 hydrin . 



Cahn, T., J. Houget, and R. Agid 



1948. The determination of glycerophos- 

 phoric acid. Application to the case of 

 the phosphatides . Bulletin. Societe 

 chimique de France, pp 666-668. 

 Chemical Abstracts, 42: 8860d(1948). 

 Phosphatides are saponified, unsaponifi- 

 ables are removed, the solution is acidi- 

 fied, the fatty acid is removed, glycero- 

 phosphoric acid is precipitated as the Ba 

 salt, and P is determined. Unsaponifiable 

 matter, fatty acids, and glycerophosphoric 

 acid may all be determined on a 0.5 to 1.0 

 g. sample by this method. 



Burmaster, C. F. 



1946. The microdetermination of serine 

 and ethanolamine in phospholipide hydro - 

 A lysates. Journal of Biological Chemis - 



try, 165: 1-6. 



Ethanolamine and serine are determined 

 by microdiffusion of the ammonia produced 

 by periodate in a solution nearly saturated 

 with potassium metaborate. Cephalin nitro- 

 gen can be measured by this method in the 

 presence of all the known components of 

 phospholipid acid hydrolysates . It is a more 

 specific measure of cephalin than the HNO2 

 method of Van Slyke (Journal of Biological 

 Chemistry, 16:121, 1913-14). No indication 

 was found of the reaction of hydroxylamine 

 with periodate to form NH3 which Nicolet 

 (Journal of the American Chemical Society , 

 6J.: 1615, 1939; Journal of Biological Chem- 

 istry , 139: 687, 1941) described. 



Obtained a better yield than Ramsey (Bio- 

 chemical Journal , 35: 39, 1941), since Ram- 

 sey's addition of K2CO3 to acid solution 

 causes generation of heat and evolution of 

 CO2 and some destruction of NH3 by the hot 

 acid periodate . 



Burness, A. T. H. andH. K. King 



1958 . Detection of fatty acids on paper 

 ^ chromatograms by means of ninhydrin. 



Biochemical Journal, 68: 32P. 

 Ethylamine or ammonia is used in the 

 developing solvent, and the salts formed 

 with the fatty acids are detected by spray- 



Candela, A., P. Capella, andG. Jacini 

 1956. Researches on phosphatidic 

 acids. III. Olii miner ali, grassi e 

 A saponi, colori e vernici, 33 : 99-101. 



Chemical Abstracts, 50:16887g (1956). 

 Choline, in 65 . 5% yield, was obtained by 

 treatment of egg lecithin with carrot enzymes 

 and a buffer, acidification of the solution 

 with HCl, and extraction with Et20. 



Cannon, J. A., K. T. Zilch, andH. J. Dutton 

 1952. Countercurrent distribution of 



if methyl esters of higher fatty acids. 



• Analytical Chemistry , 24: 1530-1532. 



The methyl esters of the higher fatty 

 acids were separated by countercurrent 

 distribution in a pentane -hexane vs 

 nitroethane-nitromethane solvent system. 

 Applicability of the method is discussed. 



Carayon-Gentil, A., andE. Corteglana 



1942. Phosphoaminolipides of brain tis- 

 sue. Fractionation and estimation of 

 i choline -containing substances. Bulletin 



de la Societe de chimie biologique , 24 : 

 89-96. Chemical Abstracts, 38:6312^ 

 (1944). 

 Free choline, lecithin and cephalin, and 

 sphingomyelin are obtained from brain tis- 

 sue by selective extraction with Me2C0, pe- 

 troleum ether, and MeOH-CHCl3 (3:1), re- 

 spectively. The extracts are hydrolyzed 

 with boiling alcoholic HCl and choline is es- 

 timated by acetylation and estimation by 



11 



