physiologlca Scandinavica, 18 (suppl . 



63): 1-189. 

 Metiiods are described for the determina- 

 tion of total lipids (gravimetric), phospho- 

 lipids (modified Fiske-Subbarow), choline, 

 ethanolamine, amino nitrogen, glycerol, 

 hexose, inositol, and cholesterol. 



Breusch, F. L. and E. Ulusoy 



1946. Isolation and identification of fatty 

 ^ acids as bis-(p-dimethylaminophenyl)- 



J ureides. Archives of Biochemistry , 11 : 



• 489-498. 



Free fatty acids are isolated from fat mix- 

 tures in approximately 80% yield by precipi- 

 tation as ureides from ether solution with 

 bis-(p-dimethylaminophenyl)-urea . The 

 properties, melting points, and solubilities 

 of the ureides of various fatty acids are 

 given. 



Brown, F . 



1950. Separation of the lower fatty acids 

 ^ as anions by paper chromatography. 



# Biochemical Journal, 47: 598-600. 

 The lower fatty acids were separated by 



paper chromatography as their Na salts us- 

 ing n-butanol as developer in the presence 

 of ammonia. The spots are revealed by 

 spraying with a solution of bromothymol blue . 



Brown, H. H., A. Zlatkis, B. Zak, and A. J. 

 Boyle 



1954. Rapid procedure for determination 

 _ of free serum cholesterol. Analytical 



Chemistry, 26: 397-399. 

 AICI3 or A1(0H)3 is used to gather digiton- 

 ide precipitate and the color produced with 

 the ferric chloride reagent of Zlatkis, Zak, 

 and Boyle (Journal of Laboratory and Clini- 

 cal Medicine , 41 : 486, 1953) is measured 

 colorimetrically . 



Brown, M., D. A. Yeadon, L. A. Goldblatt, and 

 J. W. Dieckert 



1957. Chromatography of phospholipids 

 and related compounds on glass paper 

 impregnated with silicic acid. Analyt - 

 ical Chemistry, 29: 30-31. 

 A method for the separation and identi- 

 fication of phospholipids and some of their 

 cleavage products is described. A phenol. 



• 



ethyl ether, acetone, and water solution is 

 used to develop the chromatogram on a 

 silicic acid-impregnated glass paper. Char- 

 ring with sulfuric acid is- used to locate 

 spots on the paper. (Ethanolamine and cho- 

 line chloride are not detectable by this tech- 

 nique.) A modified Dragendorf reagent 

 (Bregoff, et al. Journal of Biological Chem - 

 istry, 205: 565, 1953) or phosphomolybdic 

 acid reagent (Levine and Chargaff, Journal 

 of Biological Chemistry , 192 : 465, 1952) is 

 used for identification of quaternary ammo- 

 nium compounds, and Ninhydrin reagent for 

 detection of primary amino compounds . 



Briickner, J. 

 1941. 



Estimation of cerebrosides . Zeit- 



schrift fijr physiologische Chemie , 268: 

 163-170. Chemical Abstract^ 35:7436^ 

 (1941). ~ 



Cerebrosides are hydrolyzed with H2SO4, 

 the fatty acids are removed with CHCI3, and 

 galactose is determined using the orcin rea- 

 gent. Cerebrosides are calculated from the 

 amount of galactose. 



Buchanan, M. A. 



1959. Paper chromatography of the satu- 



■^ rated fatty acids . Analytical Chemistry , 



• 31: 1616-1618. 



A method is described for chromatography 

 of fatty acids on paper treated with mineral 

 oil. The chromatogram is developed at 37'C 

 with acetic acid - 88% formic acid - 30% 

 hydrogen peroxide (6: 1: 1) for separation of 

 the even-numbered straigjit-chain saturated 

 fatty acids from lauric to lignoceric acid in 

 the presence of large amounts of unsaturated 

 acids. Mercuric acetate and s-diphenylcar- 

 bazide were used for locating spots on the 

 chromatogram . 



BuUiard, H., I. Grundland, andM. Maillet 

 1950. Histochemical detection of cell 

 phosphatides. Compte rendu hebdoma- 

 daire des seances et memoires de la 

 ^ Societg de biologie, 144: 192-194. 



Chemical Abstracts, 44:100241(1950). 

 Phosphatides were detected in tissue sec- 

 tions by soaking the formalin -fixed tissue 

 in CdCl2> washing in acetone, and convert- 

 ing the Cd which had combined with the leci- 



10 



