teins. A micromethod based on zone 

 electrophoresis and cholesterol esti- 

 mation. Biochemical Journal, 58: 680- 

 685. 

 A method is described for the separation 

 of serum lipoproteins by paper electro- 

 phoresis. Protein was located by staining 

 with bromophenol blue. Cholesterol was 

 extracted with acetone -ethanol (1: 1) and 

 estimated by the Liebermann-Burchard re- 

 action . 



Boyd, M. J. andM. A. Logan 



1942. Colorimetric determination of 

 serine. Journal of Biological Chem- 

 istry, 146: 279-287. 

 The formaldehyde formed by the action of 

 periodate on serine is quantitatively distill- 

 ed from the reaction mixture, condensed 

 with 1, 8-dihydroxynaphthalene-3, 6-disul- 

 fonic acid, and measured colorimetrically. 

 Required 1-5 mg. serine. Error 1-2%. 



Bradbury, R. G. 



1951. Mic redetermination of glycerol. 

 Mikrochemie (vereinigt mit Mikro- 

 • chemica Acta), 38: 114-119. Chemi- 



cal Abstracts, 45:6857c (1951). 

 Glycerol is freed from the fat by hydro - 

 lyzing with KOH and converted to isopropyl- 

 iodide by reaction with HI in the presence 

 of propionic acid. TTie I2 freed during the 

 reaction is collected in a solution of Br and 

 sodium acetate in acetic acid, which oxi- 

 dizes the I to IO3-. Excess Br is removed 

 from the solution and I is freed and titrated 

 with Na2S203 . 



Bragdon, J. H. 



1951. Colorimetric determination of 



^ blood Lipides. Journal of Biological 



Chemistry, 190 : 513-517. 

 A modification of Bloor's dichromate 

 oxidation method (Journal of Biological 

 Chemistry, 170: 671, 1947). K2Cr207 in 

 H2 SO4 is used in place of Ag2Cr207, as 

 Ag may contain CI which would interfere 

 with, colorimetric measurements. Better 

 oxidation is obtained, as well as a straight - 

 line curve of optical density. 



Bragdon, J. H. 



1956. Lipid nomenclature (recommenda- 

 tions regarding the reporting of serum 

 lipids and lipoproteins). American so- 

 ciety for the study of arteriosclerosis. 

 Circulation Research, 4: 129. 

 The following are recommended: 



1. The factor 1.67 be used to convert 

 weight of cholesterol present in esterified 

 form into weight of cholesterol ester. (Kel- 

 sey and Longenecker, Journal of Biological 

 Chemistry, 139: 727, 1949). 



2 . The ratio of free cholesterol to total 

 cholesterol be expressed as a decimal. 



3 . The factor 25 be used to convert 

 lipid phosphorus to phospholipid. 



4. The amount of phosphorus in a 

 Bloor extract be considered the correct 

 lipid phosphorus content of serum . (Van 

 Slyke,. Journal of Biological Chemistry , 200: 

 525, 1953). 



5. Relative amounts of cholesterol and 

 phospholipid be expressed as the ratio of 

 total cholesterol to phospholipid, as a deci- 

 mal . Values used should represent weights, 

 not moles. 



6. Triglycerides and fatty acids be re- 

 ported as triglycerides, which predominate. 

 The term "neutral fat" be avoided or spe- 

 cifically defined. Triglycerides be report- 

 ed in milligrams. The term millequivalents 

 be confined to values for fatty acids deter- 

 mined by titration when additional determi- 

 nations are not available for the calculation 

 of triglycerides. 



Brand, F . C . and W . M . Sperry 



1941. The determination of cerebro- 

 sides. Journal of Biological Chemis- 

 try , 141 : 545-553. 

 A modification of the Miller and Van Slyke 

 (Journal of Biological Chemistry, 114: 583, 

 1936) method for sugar determination. The 

 cerebrosides are hydrolyzed, the freed sug- 

 ars are oxidized with ferricyanide,. and the 

 ferrocyanide formed is titrated with eerie 

 sulfate . 



Brante, G. 



• 1949. Studies on lipids in the nervous 

 A system with special reference to quan- 



■ titative chemical determination. Acta 



