ether residue gave more consistent and 

 slightly higher phospholipid values . 



Boyd, E. M. 



1933 . A differential lipid analysis of 

 ^ blood plasma in normal young women 



by microoxidative methods . Journal 

 " of Biological Chemistry, 101 : 323-336. 



The oxidative procedure is recommended 

 for determination of various lipids in the 

 same extract as it eliminates the possibil- 

 ity of summation of errors by using differ- 

 ent methods. 



Bloor's method ( Journal of Biological 

 Chemistry, 77: 53, 1928) is used to deter- 

 mine total fatty acids after saponification 

 of the lipids (99% recovery of known solu- 

 tions and reproducibility of + 2%), and mod- 

 ifications of Yasuda's method (Journal of 

 Biological Chemistry, 92: 303, 1931) for 

 oxidation of cholesterol digitonide (stock 

 determinations reproducible + 2%). Con- 

 firms observations that 1/2 hour heating 

 is necessary for complete oxidation. 

 Boyd's modifications (Journal of Biological 

 Chemistry, 91_: 1, 1931) of Bloor's method 

 are used for phospholipid determination. 

 Free cholesterol is estimated after remov- 

 al of phospholipid. The method checks 

 within experimental error with values from 

 direct precipitation from alcohol-ether ex- 

 tract (96% recovery with + 4.5% agreement 

 on duplicates). 



Boyd found difficulty in Okey's method 

 ( Journal of Biological Chemistry, 88: 36 7^ 

 1930) for oxidative determination of choles- 

 terol as the digitonide with respect to repro- 

 ducibility and accuracy. Phospholipids are 

 saponified with NaOH before determination 

 of iodine numbers of phospholipid fatty acids, 

 as direct determinations on whole phospho- 

 lipid according to Yasuda (Journal of Biolog- 

 ical Chemistry, 94: 401, 1931-2) gave erra- 

 tic and variable results . 



Boyd, E. M. 



1936. The extraction of blood lipids. 

 » Journal of Biological Chemistry , 114 : 



223-234. 

 Effects of variables on extraction of 

 blood lipids with Bloor's extract (alcohol - 

 ether, 3:1) were studied. 



It was found that when the extracts of 

 blood are sufficiently diluted (at least 20: 1) 

 extraction is complete. 



Boyd, E. M. 



1936. The extraction of lipids from the 

 -^ red blood cells . Journal of Biological 

 A Chemistry, 115 : 37-45. 



The effects of variables on extraction of 

 blood cell lipids were studied and are dis- 

 cussed. 



Incompletely dried acetone used in MgCl2 

 precipitation gave low phospholipid values . 



Boyd, E. M. 



1937. The storage of lipid extracts. 

 Journal of Biological Chemistry , 121: 

 485-496. 



A decrease of phospholipid during 1-3 

 months of storage was observed. After 3-6 

 months of storage a decrease in cholesterol 

 esters, total cholesterol, and total lipid, in- 

 crease in "neutral fat" and free cholesterol, 

 and no appreciable change in total fatty acids 

 was observed. Changes were generally un- 

 affected by storage conditions of light, tem- 

 perature (refrigerator or room), or solvent 

 (EtOH or EtOH-Petr. ether). See also: 

 Halliday Oournal of Nutrition, 16:285, 1938). 



Boyd, E. M. 



1937. The oxidative micro-estimation of 

 ^ plasma total lipid. Journal of Labora - 

 * tory and Clinical Medicine, 22: 956-959. 



The factor of 3.25 is proposed in place of 

 the factor of 3.7 used by Bloor for conver- 

 sion to total lipid value of the total fatty acid- 

 total cholesterol titration with potassium di- 

 chromate. 



Boyd, E . M , 



1938. The oxidative micro -estimation of 

 blood lipids. American Journal of Clin - 



9 ical Pathology. Technical Supplement, 



2: 77-90. 

 Lipids are extracted with alcohol-ether 

 (3:1), oxidized with Nicloux reagent and 

 K2Cr207, and unreacted dichromate is ti- 

 trated. 



Boyd, G. S. 



■^ 1954. The estimation of serum lipopro- 



