Bohm, P. andG. Richarz 



1954. Determination of inositol in phos- 

 phatides. Zeitschrift fiir physiolog- 

 A ische Chemie, 298 : 110-120. Chi^ - 



ical Abstracts, 49:4462d (1955). 

 Lipid material is hydrolyzed with HCl 

 and the hydrolysate is chromatographed on 

 paper. The separated inositol is extracted 

 with water, oxidized with periodate, and 

 measured iodometrically, 



Boldingh, J. 



1950. Fatty acid analysis by partition 

 chromatography. Recueil des travaux 

 ■^ chimiques des Pays-Bas et de la Belgi- 



• que, 69: 247-261 (in English). Chem - 

 ical Abstracts, 44:6348h (1950). 



A method is described for separation of 

 C5-C18 saturated n-fatty acids from their 

 mixtures and hydroxy fatty acids from satu- 

 rated n-fatty acids by chromatography on a 

 rubber column. 



Boldingh, J. 



1953 . Separation of fatty acids by chro- 

 matography. Koninkl. Vlaam. Acad. 

 Wetenschap . , Letter . en Schone 

 » Kunsten Belg., Kl. Wetenschap., 



^ Intern. CoUoquim Biochem . Problem 



Lipiden, Brussels , p. 64-81 (in Eng- 

 lish). Chemical Abstracts, 49:2097h 

 (1955). 

 Fatty acids were separated on a column 

 of rubber powder swollen with peanut oil,, 

 with acetone -water -peanut oil as mobile 

 phase . 



Borgstrom, B. 



1952. Investigation on lipid separation 

 ■ic methods. Separation of phospholipids 



# from neutral fat and fatty acids . Acta 

 ^ physiologica Scandlnavica , 25: 101-110. 



Acetone-MgCl2 precipitation gives good 

 separation of phospholipids, with about 1% 

 of the phospholipids dissolving in the solu- 

 tion. MgO chromatography gives phospho- 

 Upid-free neutral fat, but poorer purity of 

 phospholipid, and cannot separate free 

 fatty acids from choline -containing phospho- 

 lipids. Non -choline -containing phospho- 

 lipids are not recoverable unchanged. Silic- 

 ic acid chromatography is slower than 



acetone -MgCl2 precipitation, but gives 

 best all-around results; neutral fat and free 

 fatty acids are eluted quantitatively with 

 CHCI3, and phospholipids with MeOH. 



Borgstrom, B. 



1952. Investigation of lipid separation 

 . methods. Separation of cholesterol 



^ esters, glycerides, and free fatty acids . 



_ Acta physiologica Scandlnavica, 25: 



111-119. ~ 



Silicic acid chromatography is used for 

 separation of cholesterol esters from glyc- 

 erides and free fatty acids. Free fatty 

 acids are separated from glycerides in ab- 

 sence of lower glycerides by extraction of 

 the acids from a petroleum ether solution 

 with alkaline 50% ethanol . In the presence 

 of lower glycerides which would be extract- 

 ed into the alcoholic solution, IRA -400 ion 

 exchanger is used to separate the free fatty 

 acids from the glycerides . 



Borgstrom, B. 



1954. Investigation on lipid separation 

 . methods. III. Separation of tri-, di-, 



_ 1-mono-, and 2-monoglycerides. Acta 



physiologica Scandlnavica , 30: 231-239. 

 In the method described, tri-, di-, and 

 monoglycerides are separated from their 

 mixtures by chromatography on silicic acid 

 (2-monoglycerides are partially isomerized). 

 1-mono- and 2-monoglycerides are separa- 

 ted from tri- and diglycerides by partition 

 chromatography with heptane and 80% aque- 

 ous ethanol as phases. After oxidation of 

 1 -monoglycerides with periodic acid, the 

 2-monoglycerides can be isolated by chro- 

 matography on silicic acid. 



Boyd, E. M. 



1931. Low phospholipid values in dog 

 plasma . Journal of Biological Chem - 

 istry, 91: 1-12. 

 A modification of Bloor's oxidation meth- 

 od (Journal of Biological Chemistry, 82: 273, 

 1929) for use in determination of low phos- 

 pholipid levels. Essentially the same phos- 

 pholipid values were obtained with or with- 

 out heat in the alcohol -ether extraction of 

 plasma. Substitution of ethyl ether for pe- 

 troleum ether in extraction of the alcohol- 



