Beveridge, J. M. R. andS. E. Johnson 



1949. The determination of phospho- 

 lipid phosphorus. Canadian Journal 



^ of Research, 27E: 159-163. 



Phospholipid is digested with H2SO4 and 

 color is developed with molybdate -hydra- 

 zine sulfate reagent (Boltz and Mellon, In- 

 dustrial and Engineering Chemistry, Analyt - 

 ical Edition, 19: 873, 1947) and read at 830 

 mu . Accuracy of the method is within 1% 

 on a 20 ug. sample of phosphorus. 



Black, S. 



1949. A microanalytical method for the 

 volatile fatty acids . Archives of Bio- 



• chemistry , 23: 347-359. 



The volatile fatty acids are determined 

 by microdiffusion from an acidified solu- 

 tion into an alkaline solution, and micro- 

 titration of the excess alkali. Range: 

 0.2-2.0 "equivalents; error: <0.03uequiv- 

 alent . 



Blankenhom, D. H. andE. H. Ahrens, Jr. 



1955. Extraction, isolation, and identi- 

 1 fication of hydrolytic products of tri- 



^ glyceride digestion ui man. Journal 



of Biological Chemistry, 212: 69-81. 

 A description of methods for extraction 

 of lipids from intestinal contents and sepa- 

 ration into fatty acid, bile acid, and mono-, 

 di-, and triglyceride fractions. 



BU^, E. G. and W. J. Dyer 



1959. A rapid method of total lipid ex- 

 . traction and purification . Canadian 



_ Journal of Biochemistry and Physiology , 



• 37: 911-917. 



A simplification of the method of Folch, 

 et al, (Journal of Biological Chemistry, 191: 

 833, 1951). Lipids are extracted by homo- 

 genization of tissue with CHCl3-MeOH in 

 such proportions that a miscible system is 

 formed with the water in the tissue. The 

 homogenate is separated into a chloroform 

 layer containing the lipids and a methanoUc 

 layer containing the non-lipid material by 

 addition of chloroform and water . Extrac - 

 tion of the lipids is essentially complete. 



Bloor, W. R. 



1916. The determination of cholesterol 

 in blood. J ournal of Biological Chem - 

 " istry, 24: 227-231." 



Blood is extracted with alcohol -ether 

 (3: 1), the extract is evaporated to dryness 

 and the residue is extracted with chloro- 

 form. Cholesterol is then determined 

 colorimetrically using the Liebermann-Bur- 

 chard color reaction. 



Bloor, W. R. 



1928. The determination of small amounts 

 ^ of lipid in blood plasma . Journal of 



Biological Chemistry, 77: 53-73. 

 Nicloux reagent (Ag2Cr207 in H2SO4) 

 is used for oxidation of the extracted lipid, 

 and the excess dichromate is titrated with 

 thio sulfate. 



Bloor, W. R. 



1929. The oxidative determination of 

 phospholipid (lecithin and cephalin) 



A in blood and tissues. Journal of Bio - 



logical Chemistry , 82: 273-286. 

 The phospholipids are separated by pre- 

 cipitation with acetone -MgCl2 and determin- 

 ed by oxidation with Nicloux reagent and 

 titration of excess dichromate with thiosul- 

 fate. 



Bloor, W. R. 



1947. A colorimetric procedure for de- 

 _ termination of fatty acids . Journal of 



Biological Chemistry , 170 : 671-674. 

 Fatty acids are determined-by colorimet- 

 ric measurement of the color change pro- 

 duced by oxidation with Nicloux reagent. A 

 special colorimeter Is required. 



Bohm, P., S. Dauber, andL. Baumelster 



1954. Neuraminic acid, its occurrence 

 and its determination in serum . Klin- 

 ische Wochenschrift , ^: 289-292. 

 Chemical Abstracts, 48:7674e (1954). 

 Serum protein is precipitated with tri- 

 chloracetic acid, centrifuged, and washed 

 with water. The precipitate is treated with 

 Bial reagent and heated, and the red-violet 

 reaction product of neuraminic acid is ex- 

 tracted with amyl alcohol and read at 570 mu. 



