colorimetric determination of phos- 

 phate. Biochemical Journal, 32 : 295- 

 298. 

 Organic material is digested with HCIO4 

 and the phosphorus is converted to phospho- 

 molybdate. The phosphomolybdate is ex- 

 tracted into isobutyl alcohol, reduced with 

 SnClo* and the color is read colorimetri- 

 cally. The method is not subject to most 

 of the interferences which affect the meth- 

 ods of Fiske and Subbarow and Kuttner and 

 Cohen. The standard method will deter- 

 mine 1-lOO^g. of phosphorus, and the 

 micro method 0.1-lOug. 



Bergmann, W. 



1940. The splitting of digitonides . 

 ■ Journal of Biological Chemistry, 132 : 



471-472 . 

 Digitonides are split with dry pyridine, 

 the pyridine is distilled off, and the digit- 

 onin is extracted with ether. The residue 

 is steroid in yield of over 



Bergstrbm, S. and K. Paabo. 



1954. A method for the separation of 

 _. saturated and monoun saturated fatty 



^ acids through hydroxylation . Acta 



chemica scandinavia, 8: 1486-1487. 

 The fatty acids are hydroxylated with 

 performic acid, methylated, and separated 

 by elution from a silicic acid column. 



Berk, L. C, N. Kretchmer, R. T. Holman, 

 and G. O. Burr 

 1950. Microdetermination of uns'atu- 

 rated fatty acids by alkali isomeriza- 

 * tion. Analytical Chemistry, 22:718- 



720. 

 Aqueous alkali and high temperature and 

 pressure are used for isomerization of un- 

 saturated fatty acids . The fatty acids are 

 then determined by ultraviolet spectro- 

 photometry. A discussion of the method 

 and data for linoleic, linolenic, and 

 arachidonic acids are presented. 



Bertram^ S. H. and R. Rutgers 



1938. The estimation of glycerol and 



and some other hydroxylated compounds . 

 Recueil des travaux chimiques des 

 Pays -Bas et de la Belgique , 57: 681-687. 



Chemical Abstracts, 32:78575(1938). 

 The copper complex formed with glyc - 

 erol in alkaline solution is acidified, KI is 

 added, and the excess is titrated with thio- 

 sulfate . 



Best, M., E.J. VanLoon, J. D. Wathem, and 

 A.J. Seger 

 1954. Comparison of serum cholesterol 

 methods. American Journal of Medi - 

 ■ cine, 16: 601. 



Serum cholesterol was determined by 

 the methods of Schoenheimer-Sperry (Jour - 

 nal of Biological Chemistry, 106 : 745, 1934); 

 Abell, et al (Journal of Biological Chemistry , 

 195 : 357, 1952); Gershberg-Forbes (Jour - 

 nal of Laboratory and Clinical Medicine, 27 : 

 1439, 1942); modified Pearson, et al ( Analyt - 

 ical Chemistry, 25: 813, 1953); and Zlatkis, 

 Zak, and Boyle (Journal of Laboratory and 

 Clinical Medicine , 41: 486, 1953). The Abell, 

 et al, and Schoenheimer-Sperry methods 

 agreed closely. When compared to the Abell 

 method, the values of the Gershberg-Forbes, 

 Pearson, and Zlatkis methods were higher 

 by 15, 35, and 90 mg. percent, respective- 

 ly. Since an alcohol -acetone extract of se- 

 rum yields similar results by the Schoen- 

 heimer-Sperry and^Zlatkis methods while 

 the Zlatkis method yields values of 90 mg. 

 percent higher when used directly on the 

 same serum, it is suggested that the Zlatkis 

 method measures a non-extractable lipid of 

 protein as well as cholesterol. 



Bevan, T. H., G.I. Gregory, T. Malkin, and 

 A. G. Poole 

 1951. Chromatographic separation of 

 . choline -containing phospholipids from 



. phospholipid mixtures. Journal of the 



Chemical Society, 841-842. 

 Phospholipids were separated by chroma- 

 tography on cellulose columns or on paper 

 using a chloroform, water, and ethanol mix- 

 ture as solvent. Ethanolamine- and serine- 

 containing phospholipids were located on the 

 developed chromatogram with ninhydrin, and 

 choline -containing phospholipids were located 

 with phosphomolybdic acid- stannous chloride 

 reagent. Choline phospholipids were obtain- 

 ed uncontaminated. 



