Delsal (Bulletin de la Soci^t6 de chimie bio- 

 logique , 24: 296, 1942), and Abell (Journal 

 of Biological Chemistry, 195 : 351, 1952) 

 methods for cholesterol determination. 

 The S-S and Abell methods were found to 

 be superior to the other two methods with 

 regard to the standard error inherent in 

 the method. 



Barron, E.J. and D. J. Hanahan 



1958. Observations on the silicic acid 

 chromatography of the neutral lipids 



1 of rat liver, beef liver, and yeast. 



Journal of Biological Chemistry , 231: 

 493-503. 

 Pigm.ents and hydrocarbons were eluted 

 from a column of silicic acid with hexane, 

 sterol esters with 15% benzene in hexane, 

 triglycerides and free fatty acids with 5% 

 ether in hexane, free sterols with 15-20% 

 ether in hexane, diglycerides with 30% 

 ether in hexane, and monoglycerides with 

 90-100% ether in hexane. 



Barry, G. T., Y. Sato, andL. C. Craig 



1951. Distribution studies. XIII. Sep- 

 . aration and estimation of the higher 



^ normal fatty acids . Journal of Biologi - 



* cal Chemistry, 188: 299-306. 



C5 to C^g normal fatty acids were sepa- 

 rated by counter -current distribution in 

 isopropyl ether and 1 M potassium phosphate 

 and estimated by titration . 



Bartlett, G. R. 



1959. Phosphorus assay in column chro- 

 matography. Journal of Bi ological Chem- 



^ istry, 234: 466-468. 



A modified Fiske-Subbarow method which 

 uses heating of the phosphorus reaction mix- 

 ture in H2SO4 to produce an increase in 

 light absorption at 830 mp.. This method 

 permits greater latitude in reagent concen- 

 trations. The color developed is stable for 

 24 hours . 



Bauer, F. C, Jr. and E . F. Hirsch 



1949. A new method for the colorimetric 

 determination of the total esterified 



# fatty acids in human sera. Archives 

 of Biochemistry , 20: 242-250^ 



Fatty acid esters are converted to 



hydroxamic acids and determined as col- 

 ored ferric salts. A discussion of the pro- 

 cedure and sources of error is included. 



Beadle, G. W. 



1944. An inositolless mutant strain of 



neurospora and its use in bioassays . 



Journal of Biological Chemistry, 156: 



683-689. 

 A method is described which is suitable 

 for use with concentrations of inositol be- 

 tween 5 and 30 itg.in 20 ml. of medium . A 

 mutant strain of Neurospora crassa which 

 requires inositol for growth is used in the 

 bioassay. 



Beattie, F. J. R. 



1936. A colorimetric method for the de- 

 termination of choline and acetyl- 

 choline in small amounts. Biochemical 

 Journal, 30: 1554-1559. 

 Choline and acetylcholine are precipitated 

 as their reineckates. The reineckate is dis- 

 solved in acetone and measured colorimetri- 

 cally. 



Beerthuis, R. K., G. Dijkstra, J. G. Keppler, 

 and J. H. Recourt 



1959. Gas -liquid chromatographic anal- 

 ysis of higher fatty acids and fatty- 

 •h acid methyl esters. Annals of the New 



# York Academy of Science, 72 (art. 13): 



616-632. 



Fatty acids were separated by gas chro- 

 matography and the separated acids at the 

 exit of the column were combusted over CuO 

 at 1000° and the evolved CO2 was meas- 

 ured katharometrically. 



Benham, G. H. and L. Klee 



1950. An improved method for the de- 

 termination of iodine numbers . Jour - 

 nal of the American Oil Chemists 

 Society, 27: 127-129. Chemical Ab- 

 stracts , 44:5119c (1950). 

 A modification of the method of Rosen- 

 mund and Kuhnhenn (Ber . 56: 1262, 1923). 

 The use of Hg acetate permits a determina- 

 tion to be done in 1 minute. 



Berenblum, I. andE. Chain 

 A 1938. An improved method for the 



