ings of the Society of Experimental Bio- 

 logy and Medicine,41: 5 14, 1939) of precip- 

 itation with "colloidal iron" and magnesium 

 sulfate. 



The values that were obtained indicated 

 that extraction of the phospholipids by all 

 the above methods was probably complete, 

 but washing procedures yield final products 

 of slightly different compositions. The 

 Folch and Van Slyke procedure was found 

 to be reliable for routine analyses . 



Artom, C. 



1945. A quantitative method for ethanol- 

 amine and serine as a basis for the de- 

 termination of phosphatidyl ethanolamine 

 A and phosphatidyl serine in tissues. Jour- 



nal of Biological Chemistry, 157: 585- 

 594^ 

 The method is based on the reaction of an 

 ethanolamine -serine mixture with alkaline 

 periodate before and after ethanolamine is 

 removed by adsorption on Permutit. The 

 NH., produced by the reaction is steam dis- 

 tilled and determined. Conditions for use 

 and limitations of the method are discussed. 



Ashley, B. D. andU. Westphal 



1955. Separation of small quantities of 

 . saturated higher fatty acids by reversed- 



^ phase paper chromatography. Archives 



of Biochemistry and Biophysics, 56: 1-10. 

 Fatty acids were separated on paraffin oil- 

 er latex-coated paper with aqueous methanol 

 saturated with cyclohexane or trimethylpen- 

 tane, and aqueous methanol saturated with 

 paraffin oil, respectively. Spots were detec- 

 ted by spraying with bromothymol blue, or 

 immersing in a solution of lead acetate and 

 developing the spots as lead sulfide or rho- 

 dlzonate. Separates 10-50/ig. of C.^-C^^ 

 acids . 



Awe, W . and B . Grote 



1958. Paper chromatography of thiocyano- 

 . gen derivatives of fatty acids. Fette, 



J Seifen, Anstrichmittel, ^: 806-809. 



* Chemical Abstracts, 53: 3737h (1959). 



Fatty acid cyanogen derivatives are sepa- 

 rated by paper chromatography using an 

 acetic acid-undecane solvent system. The 

 separated derivatives are located by spray- 



ing the dried chromatogram with an 

 FeNH4(S04)2 or FeCl3 solution in an ammo- 

 nia atmosphere. 



Axelrod, J., J. Relchenthal, andB. B. Brodie 

 1953. The direct determination of phos- 

 phatidyl ethanolamine and phosphatidyl 

 serine in plasma and red blood cells. 

 Journal of Biological Chemistry , 204: 

 903-911. 

 Ethanolamine and serine are determined 

 by spectrophotometric measurement of their 

 colored dinitroflourobenzene derivatives. 



It was found that sensitivity of the Fiske 

 and Subbarow phosphorus determination 

 ( Journal of Biological Chemistry , 66 : 375, 

 1925) was increased 4-fold by heating 10 

 minutes in a boiling water bath after addition 

 of reagents. 



Ayers, C. W. 



1956. Estimation of the higher fatty acids 

 ^ Cy-CjQ. Analytlca Chlmica Acta 15: 



• 77-83. ~ 

 Methods are described for estimation of 



the Cy-Cjg fatty acids as their copper or co- 

 balt soaps. The soaps are dissolved in chlo- 

 roform and the optical density of the solution 

 is measured at 675 m/j. for copper or 525 m^. 

 for cobalt. 



Ballance, P. E. and W. M. Crombie 



1958. Paper chromatography of saturated 

 -^ and unsaturated fatty acids . Biochem- 



# leal Journal , ^: 632-640. 



Methods and solvent systems are describ- 

 ed for reversed-phase chromatography of 

 fatty acids on paper impregnated with paraf- 

 fin oil, castor oil, or polythene. Data on the 

 separation of over 40 fatty acids are given. 



Bargeton, D., M. E. Trlcand-Redel, and P. Gros 



1959. Comparison of results from three 

 methods for the determination of serum 

 cholesterol. Revue francaise d' etudes 

 cllniques et biologiques , 4: 326-334. 

 Chemical Abstracts, 53:18143e (1959). 



A comparison of the Schoenheimer-Sperry 

 (Journal of Biological Chemistry , 106: 145, 

 1934), Grigaut ( Compte rendu hebdomadaire 

 des seances et mgmoire s de la Socigtg de 

 biologie, 112:34, 1933), Machebouf and 



