t±ie fatty acids obtained by hydrolysis of pig 

 mesenteric fat. Separations of saturated 

 or unsaturated groups was good, but over- 

 lapping occurred in mixtures containing both 

 saturated and unsaturated acids. 



Ahrens, E. H., Jr. 



1955. Application of countercurrent dis- 

 tribution for the study of lipids . 

 _. Proceedings of the International Con- 



ference on Biochemical Problems of 

 Lipids, 2nd Ghent . 

 A discussion of the applicability and use 

 of counter-current distribution for lipid 

 studies. 



Albers, R. W. andO. H. Lowry 



1955. Flourometric determination of 0. 1 

 to 10 micrograms of cholesterol. Ana- 



■ lytical Chemistry, 27: 1829-1831. 



The flourescence produced by cholester- 

 ol in a trichloroethane, sulfuric acid, and 

 acetic acid solution is used to measure the 

 cholesterol extracted from 1 to 25 ug. of 

 brain or other tissue. A stable, sensitive 

 and precise method. 



Allen, R. J. L. 



1940. The estimation of phosphorus. 

 Biochemical Journal, 34: 858-865. 

 A modification of the Fiske-Subbarow 

 reaction which uses amidol-sodium bisul- 

 fite as reducing agent . 



Anderson, D. M. W. 



1959. Applications of infrared spectro- 

 scopy: the identification and determi- 

 nation of gas -chromatographic frac- 

 tions. Analyst, 84 : 50-55. 

 Methods are described for collecting 

 the components of mixtures separated by gas 

 chromatography, and identification and quan- 

 titative determination of the components by 

 vapor-phase infra-red spectroscopy. 



Appleton, H. D., B. N. Ladu, B. B. Levy, 

 J. M. Steele, andB. B. Brodie 



1953. A chemical method for the deter- 

 mination of free choline in plasma. 

 Journal of Biological Chemistry, 205 : 

 803-813. 

 The method involves extraction of 



choline into acetone, evaporation of the 

 acetone, and removal of interfering matter 

 with butanol . After precipitation of choline 

 as enneaiodide, the precipitate is dissolved 

 in ethylene dichloride and measured spectro- 

 photometrically at 365 mp.. As little as 5 



jUg. of choline is estimated. The method ap- 

 pears to be specific when used for plasma 

 choline, but material other than choline is 



also assayed when the method is used for 

 urine or tissue analyses. Adsorbed iodine 

 does not interfere. 



Arcus, A. C. 



1959. Nephelometric detection of lipides 

 in chromatographic column effluents. 

 Analytical Chemistry, 31: 1618-1620. 

 A method is described for the rough esti- 

 mation of the quantity of lipid material in ef- 

 fluents from chromatographic columns. The 

 lipid material is precipitated from a meth- 

 anolic solution with water, and the suspension 

 is measured by nephelometry. The method 

 is not specific, and is only applicable to mate- 

 rial soluble in MeOH and insoluble in 

 MeOH-H,0 (1:2). 



Armbruster, O. andU. Beiss 



1957. Paper chromatography of the phos- 

 . phatides. Naturwissenschaften, 44: 



^ 420-421. Chemical Abstracts, 



52:10268f (1958). 

 A study of various paper-solvent com- 

 binations with regard to their suitability for 

 separation of phosphatides. Data concerning 

 those combinations found to be acceptable 

 are given. 



Artom, C. 



1941. Cephalins, choline -containing phos- 

 pholipids, and total phospholipids in 

 normal human plasma. Journal of 

 Biological Chemistry , 139: 65-70. 

 A comparison of methods of extraction 

 and purification of lipids. The methods com- 

 pared were: 



1 . Extraction with cold alcohol followed 

 by continuous hot alcohol extraction. 



2. Precipitation with ammonium sulfate 

 of the plasma proteins at pH 3, followed by 

 continuous extraction with hot alcohol- 



3 . Folch and Van Slyke method (Proceed- 



* 



