METHODS FOR LIPID ANALYSIS 



Since the early 1930's the field of lipid re- 

 search has grown tremendously. In conjunction 

 with the expansion of our knowledge of lipids, 

 many methods for the determination of these 

 constituents have been published at a pace which 

 makes it difficult for the investigator to keep 

 abreast of developments. This bibliography was 

 compiled in order that references to the many 

 methods might be brought together and made 

 more readily available. 



No effort has been made to include papers 

 wherein the method of choice is "standard" or 



only slightly modified, or those referred to in 

 the "New Methods" section of the Journal of Lipid 

 Research. Of the methods published before 1930, 

 only the classical ones have been included. The 

 papers are listed alphabetically by first author. 

 Chemical Abstract numbers are included for the 

 less common journals. 



The author wishes to thank Dr. Charles G. 

 Wilber and Mr. Paul F. Robinson of the Army 

 Chemical Center for their many helpful sugges- 

 tions . 



Key to Symbols: 



■^ Separation and purification 



# Glycerides, fatty acids and total lipids 



^ Phospholipids 

 ■ Cholesterol 



Abell, L. L., B. B. Levy, B. B. Brodie, and 

 F. E. Kendall 



1952. A simplified method for the esti- 

 mation of total cholesterol in serum 

 and demonstration of its specificity. 

 Journal of Biological Chemistry , 195 : 

 357-366. 

 Serum is treated with alcoholic KOH 

 to hydrolyze the cholesterol esters, the 

 freed cholesterol is extracted into petro- 

 leum ether, and an aliquot of the extract 

 is used for determination of cholesterol 

 by the Liebermann-Burchard color reac- 

 tion. 



It is shown by counter -current distri- 

 bution that only about 2% of the material 

 determined as cholesterol by this method 

 is other material. The Schoenheimer- 

 Sperry method produced the same results. 



Abu-Nasr, A.M. and R. T. Holman 



1954. Highly unsaturated fatty acids . 

 III. Isolation of methyl eicosapen- 

 taenoate, ethyl docosapentaenoate, 

 "k and ethyl docosahexaenoate from 



# cod liver oil esters by chromatogra- 



phy. Journal of the American Oil 

 Chemists Society , 31: 41-45. 



Chemical Abstracts, 48:4229h (1954). 

 Fatty acids from cod liver oil were 

 separated on a Darco G-60 charcoal -Hyflo 

 Supercel column using isopropanol as sol- 

 vent and methyl behenate as displacer . 



Abu-Nasr, A.M. and R. T. Holman 



1955 . ' Isomerization of polyunsaturated 

 fatty acids and their esters by sodium 

 amide in liquid ammonia . Journal of 



# the American Oil Chemists Society, 

 32: 414-418. Chemical Abstracts, 

 49:12857d(1955). 



Polyenoic acids were isomerized by us- 

 ing sodium amide in liquid ammonia, and 

 determined by ultraviolet and infrared spec- 

 trophotometry . 



Ahrens, E. H., Jr. andL. C. Craig 



1952. Separation of the higher fatty acids. 

 ■^ Journal of Biological Chemistry, 195: 



• 299-310. 



Countercurrent distributioji was used 

 to separate a synthetic mixture of lauric, 

 myristic, palmitic, and stearic acids; a 

 synthetic mixture of oleic, linoleic, and 

 linolenic acids; and an unknown mixture of 



