OUey, J. 



1955. Quantitative paper chromatog- 

 raphy of lipid constituents. Proceed- 

 , ings of the International Conference on 



Biochemical Problems of Lipids, 2nd 

 Ghent . (Pub. 1956) pp. 49-55. 

 A discussion of methods for estimation 

 of the constituents of lipid hydrolysates by 

 paper chromatography. 



Ono, F. andY. Toyama 



1943. The highly unsaturated acids in 

 fats and oils. I. Re -examination of 

 the sodium salt-acetone and lithium 

 ^ salt -acetone methods for the separa- 



^ tion of highly unsaturated acids of fish 



* oils. Journal of the Chemical Society 

 of Japan, 64: 1327-1331. Chemical Ab- 

 stracts , Jl:3755a (1947). 



The lithium salt-acetone method was 

 found to be better than the sodium salt- 

 acetone method for separation of the highly 

 unsaturated acids of fish oils . 



Ory, R. L., W. G. Bickford, and J. W. Dieckert 

 1959. Glass paper chromatography of 

 , the long-chain fatty acids, brominated 



^ derivatives, and methyl esters . Ana- 



* lytical Chemistry, 31: 1447-1448. 



A method is described for separation of 

 long-chain fatty acids, both saturated and 

 unsaturated. The methyl esters of the 

 acids are brominated and chromatographed 

 on glass paper impregnated with silicic 

 acid. Isooctane is used as the developing 

 solvent. 



Alumina paper did not give as good results. 



Page, E . and L . Michaud 



1951. The titrimetric determination of 

 _ plasma fatty acids. Canadian Journal 



* of Medical Science, 29: 239-244. 

 Plasma lipids are extracted and sapon- 

 ified, and the fatty acids are freed with HCl 

 and extracted. The fatty acids are then 

 dissolved in an alcoholic solution of thymol 

 blue and titrated with tetramethylammonium 

 hydroxide . 



Pangbom, M . C . 



1941 . A note on the purification of leci- 

 ■ic thin . Journal of Biological Chemistry, 

 A 137:545-548. 



Lecithin is precipitated with CdCl2 from 

 an alcoholic solution, and purified by re- 

 peated extraction from an 80% alcohol - 

 petroleum ether solution. Sphingomyelin 

 and plasmalogen are not removed from the 

 lecithin . 



See Pangborn, Journal of Biological Chem - 

 istry , 188: 471, 1951, for modifications of 

 the method. 



Pangborn, M. C. 



1942 . Isolation and purification of a 



, serologically active phospholipid from 



beef heart . Journal of Biological Chem- 

 istry , 143: 247-256. 

 "Cardiolipin" was extracted from beef 

 heart with methanol and purified by recrystal- 

 llzation of the CdCl2 salt from alcohol and 

 ether. It was identified as- essential mate- 

 rial for reactivity of beef heart antigens in 

 the serological test for syphilis. 



Pangbom, M . C . 



1944. Acid cardiolipin and an improved 

 method for the preparation of cardio- 



^ lipin from beef heart . Journal of Bio- 



logical Chemistry , 153 : 343-348. 

 Cardiolipin is extracted from the tissue 

 with methanol (Pangborn, Journal of Biolog- 

 ical Chemistry, 143 : 247, 1942), precip- 

 itated with BaCl2. and purified by reprecip- 

 itation from solvent solutions with BaCl2 

 and CdClo- Yield of 0.6 grams was obtained 

 from a kilogram of moist tissue . 



Pangborn, M . C . 



1945. A note on the preparation of cardio- 

 lipin . Journal of Biological Chemistry, 

 157: 691-692. 



Na2S04 is recommended for decomposition 

 of the crude barium salts of cardiolipin in 

 place of the NaCl previously used (Pangborn, 

 Journal of Biological Chemistry , 153: 343, 

 1944). The NaCl method does not give com- 

 plete recovery of cardiolipin in a large scale 

 preparation. 



53 



