Pangborn, M. C. 



1945. A simplified preparation of cardio- 

 lipin, with a note on purification of 

 A lecithin for serologic use. Journal of 



Biological Chemistry, 161: 71-82. 

 A simplification of Pangborn (Journal of 

 Biological Chemistry, 153 : 343, 1944). 



Pangborn, M. C. 



1951 . A simplified purification of leci- 



"A" thin . Journal of Biological Chemistry , 



A JJ8: 471-476. 



Lecithin is purified by repeated precip- 

 itation from alcohol with CdClo. Lecithin 

 with an N:P ratio of 1.01:1 is obtainable. 

 Yield; 10.5 gm. from 12 eggs. 



Paquot, C. 



1947. The determination of the sapon- 

 ification number of fats . Journal des 

 recherches du Centre national de la 

 # recherche scientifique (Paris) pp. 131- 



135. Chemical Abstracts, 42:5689a 

 (1948). 

 The double bonds of unsaturated aliphatic 

 acids may react during saponification of 

 triglycerides with alcoholic KOH, and lead 

 to erroneous results. 



Park, J. T. andM. J. Johnson 



1949. A submicro determination of 

 glucose . Journal of Biological Chem- 

 istry, 181 : 149-151. 

 A very sensitive method. Glucose is de- 

 termined by reduction of ferricyanide and 

 colorimetric measurement. Range of 1-9 

 /ig. of glucose in 1-3 ml. sample. The re- 

 action is not specific for glucose. -^ 



Pearson, S., S. Stem, andT. H. McGavack 

 1953. A rapid, accurate method for the 

 determination of total cholesterol in 

 ■ serum. Analytical Chemistry , 25: 813- 



814. ~ 



Glacial acetic acid, p-toluenesulfonic acid 

 and acetic anhydride are added to 0.1 ml. 

 of serum and allowed to cool without mixing. 

 Con. H9SO4 is added and the solution is 

 mixed. After 20 minutes, the optical den- 

 sity is measured at 550 m^ . Recovery of 

 added cholesterol and cholesterol acetate = 

 99.2 + 3.6%. Average variations in values 



for analyses of the same serum over a 24 

 hour period ranged from 0.4 to 2.4%. Av- 

 erage deviation of duplicates was less than 

 5%. Average deviation from values by the 

 Schoenheimer-Sperry method + 3.5%. Equi- 

 molar quantities of cholesterol and choles- 

 teryl acetate give equal color densities by 

 this method. 



Perila, O. 



1956. Separation of saturated straight 

 chain fatty acids . HI. Quantitative pa - 

 ^ per chromatography. Acta chemica 



Scandinavia , 10 : 143-144. 

 Solvent systems are described for sepa- 

 ration of the fatty acids from formic to 

 cerotic by paper chromatography. 



Peterson, M . H . and M.J. Johnson 



1948. The estimation of fatty acids of 



_. intermediate chain length by partition 



^ chromatography. Journal of Biological 



• Chemistry , 174 : 775-789. 



The fatty acids from formic to capric 

 were separated on a Celite column using 

 sulfuric acid as the stationary phase with 

 Skellysolve B-benzene and butanol-chloro- 

 form as mobile phases. Higher fatty acids 

 do not interfere. 



PhilUps, G. B. 



1958. The isolation and quantitation of 

 the principal phospholipid components 



■A" of human serum using chromatography 



▲ on silicic acid. Biochimica et Biophys- 



ica Acta, _29: 594-602. 

 A modification of the Lea, Rhodes, and 

 Stoll chromatographic method (Biochemical 

 Journal , 155 : 19, 1944). Lecithin, sphingo- 

 myelin, lysolecithin, and phosphatidyl etha- 

 nolamine were isolated from human serum 

 by chromatography on silicic acid using 

 methanol and chloroform as eluents. 



Extraction of serum with chloroform - 

 methanol (1:1) was found to be as effective 

 as Bloor's alcohol-ether (3:1) extraction. 



Phillips, G. B. andN. S. Roome 



1959. Phospholipides of human red blood 

 , cells. Proceedings of the Society for 



Experimental Biology and Medicine , 100 : 

 ^ 489-492. 



54 



