Red blood cell phospholipids were sepa- 

 rated into ethanolamine - and serine -con- 

 taining phospholipids, lecithin, sphingo- 

 myelin and lysolecithin by chromatography 

 of silicic acid. 



Pierfitte, M. and J. Barrier 



1959. Microdetermination of serum cho- 

 lesterol by a reaction with ferric chlo- 

 ride . Bulletin de la Societe de phar- 

 macie de Nancy, No . 40, pp. 31-39. 

 Chemical Abstracts, 54:1648e (1960). 

 A modification of the method of Zlatkis, 

 et al (Journal of Laboratory and Clinical 

 Medicine , 41: 486, 1953). The unmodified 

 Zlatkis method gave results approximately 

 7% too high . 



Pikaar, N. A. and J. Nijhof 



1958. Microdetermination of the fatty 

 ^ acids in blood serum . Biochemical 



* Journal, 70: 52-57. 



The lipids in 2 ml. of serum are extrac- 

 ted with ethanol-ether (3:1) and saponified. 

 The fatty acids are isomerized with KOH 

 in ethylene glycol, and the amounts of each 

 of the polyethenoid fatty acids are calcu- 

 lated from the difference in optical densi- 

 ties at various wave lengths before and 

 after the isomerization. 



Saturated fatty acids are determined by 

 a modification of the method of van de Kamer 

 ( Biochemical Journal, 61: 180, 1955). 



The method compares well with the meth- 

 od of Herb and Riemenschneider ( Analytical 

 Chemistry , 25: 953, 1953) . 



Pippen, E . L . , E.J. Eyring, and M . Nonaka 

 1957. Chromatographic separation of 

 . 2, 4-dinitrophenylhydrazones. Analyt - 



ical Chemistry, 29: 1305-1307. 

 Methods for the preparation and chroma- 

 tography on silicic acid-celite of the 2, 4-di- 

 nitrophenylhydrazone derivatives of aliphat- 

 ic aldehydes and ketones are described. 

 Data on separation of various combinations 

 of the derivatives are given. 



Piatt, B . S . and G . E . Clock 



1943. The estimation of inositol in ani- 

 mal tissues. Biochemical Journal, 37: 

 709-712. 



The ether -soluble -70% acetone -insoluble 

 fraction is separated from an aqueous ex- 

 tract of tissue. Glucose is removed from 

 the extract by yeast fermentation and acid- 

 ic and basic substances are removed by 

 ion-exchange resins. The free inositol is 

 oxidized with HIO4 and the excess acid is 

 estimated lodometrlcally . 



Pohle, W. D. and V. C. Mehlenbacher 



1950 . A modification of the periodic 

 method for the determination of mono- 

 glycerldes and free glycerol in fats and 

 # oils. Journal of the American Oil 



Chemists Society, 27: 54-56. Chemical 

 Abstracts, 44:3272a (1950). 

 Free glycerol and monoglycerldes are 

 separated by extraction of the glycerol with 

 water from a chloroform solution of the 

 sample. Glycerol is determined on the wa- 

 ter solution, and monoglycerldes are deter- 

 mined in the chloroform solution by period- 

 ic acid oxidation. 



Pollak, 0. J. and B. Wadler 



1952 . Rapid turbldlmetrlc assay of cho- 

 lesterols . Journal of Laboratory and 

 Clinical Medicine, 39: 791-794. 

 Free or total cholesterol is assayed by 

 nephelometric measurement of the turbidity 

 produced by cholesterol digitonlde . 



Polonovski, J., J. Etlenne, M. Paysant, and 

 M. Petit 



1959 . Chromatographic fractionation of 

 , plasma phospholipides. Annales de blo- 



2 logie cllnique (Paris) 17: 186-192. 



Chemical Abstracts, 53:22181e (1959). 

 A method is described for separation of 

 plasma phospholipids by paper chromatog- 

 raphy . 



Popjak, G. 



1943. Colorlmetrlc determination of to- 

 tal, free, and ester cholesterol in tissue 

 ■ extracts. Biochemical Journal, 37: 



468-470. 

 A modified combination of the Kelsey 

 ( Journal of Biological Chemistry , 127: 15, 

 1939) and Schoenheimer-Sperry (Journal of 

 Biological Chemistry, 106: 745, 1934) meth- 

 ods. Cholesterol is precipitated as the 



55 



