digitonide and separated . The digitonlde 

 is then decomposed and the cholesterol is 

 determined by colorimetric measurement 

 of the color produced with Liebermann- 

 Burchard reagent. 



Privett, O. S., E. Breault, J. B. Covell, 

 L. N. Norcia, andW. O. Lundberg 



1958. Solubilities of fatty acids and de- 

 rivatives in acetone. Journal of the 

 American Oil Chemists Society , 35 : 



• 366-370. Chemical Abstracts, 52: 



15095h (1958). 

 Solubilities of fatty acids, alcohols, tri- 

 glycerides, and methyl esters in acetone 

 were studied using purified materials. Data 

 on solubility of the materials at different 

 temperatures are given. 



Quaife, M. L., R. P. Geyer, andH. R. 

 Bolliger 



1959. Rapid paper chromatographic 



j^ microassay of free and ester choles- 



terol of blood. Analytical Chemistry, 



■ 31: 950-955. 



A method is described for separation of 

 free and ester cholesterol from blood serum 

 by chromatograpl^ of an acetone -ethyl ether 

 (1:1) extract on ZnC03 -impregnated filter 

 paper. The cholesterol -containing areas 

 are eluted with CHClo and cholesterol is 

 determined on the eluate by a modified 

 Zlatkis procedure. Effects of variables 

 and possible interferences are discussed. 

 Coefficient of variation of duplicates done 

 on different days was 3.5%. 



Radin, N. S., F. B. Lavin, and J. R. Brown 

 1955. Determination of cerebrosides. 

 Journal of Biological Chemistry , 217 : 

 789-796. 

 Interfering ions are removed by ion-ex- 

 change chromatography. Cerebrosides are 

 determined by a modified anthrone proce- 

 dure (Black, Analytical Chemistry , 23: 

 1792, 1951), after solution in phosphoric 

 acid. Average recovery is 99.7% and 

 standard deviation is 1.9%. 



Radin, N. S. and J. R. Brown 



1955. Preparative isolation of cerebro- 

 ■^ sides. Federation Proceedings, 14: 



266. 

 Lipids from beef spinal cord are mixed 

 with ether and Celite and filtered. The fil- 

 ter cake is air dried and extracted with hot 

 alcohol. The filtrate is cooled and the re- 

 sulting precipitate is filtered off, dissolved, 

 and passed through a "Flourisil" column to 

 remove the phospholipids. The eluate is dried, 

 dissolved in chloroform -alcohol-water 

 (8:10:1), and passed through an ion exchange 

 resin mixture. The resulting cerebroside 

 material assays 87% pure. 



Radin, N. S. 



1957. Glycolipide chromatography. 



Federation Proceedings , 16: 825-826. 

 In the method described, a mixture of 

 brain lipids is passed through a "Flourisil" 

 (activated magnesium silicate) column to 

 remove phosphatides and gangliosides, after 

 which the sulfatides are adsorbed on a mix- 

 ture of ion exchange resins. The cerebro- 

 sides and other non-ionic materials are 

 then chromatographed on an unsulfated Dowex 

 50 column. The sulfatides may be eluted 

 from the ion exchange column with a chloro- 

 form-alcohol-water solution of lithium ace- 

 tate and precipitated with BaCl2. 



Ramsay, W. N. M. and C. P. Stewart 



1941 . The analysis of blood phospho- 

 A lipins. Biochemical Journal, 35: 39-47. 



The amounts of lecithin, sephalin, and 

 sphingomyelin in blood are calculated from 

 the analysis for phosphorus, choline, glyc- 

 erol, and ethanolamine . Choline is deter- 

 mined as the reineckate, and glycerol as 

 formaldehyde . 



Ramsey, L.L. andW. I. Patterson 



1945. Separation and identification of 

 the volatile saturated fatty acids (C^ to 

 -^ C4). Journal of the Association of Of- 



ficial Agricultural Chemists , 28: 644- 



656. 

 A method is described for separation of 

 the C1-C4 saturated fatty acids by chroma- 

 tography on silicic acid. Tlie fatty acids 

 are eluted with butanol -chloroform, using 

 water as the stationary phase, and identi- 

 fied by microscopic examination of their 

 crystalline derivatives. n-Butyric and 



56 



