Sato, Y., G. T. Barry, and L. C. Craig 



1947. Identification of small amounts of 

 . organic compounds by distribution 



_ studies. Vn. Separation and estima- 



tion of normal fatty acids . Journal of 

 Biological Chemistry, 170 : 501-507. 

 Counter-current distribution is used for 

 the separation and quantitative estimation 

 of the C2-C5 normal fatty acids to within 

 2-3%. 



Schaffer, F. L., J. Feng, and P. I. Kirk 



1953. Microgram and submicrogram de- 

 termination of phosphate. Analytical 

 ^ Chemistry , 25: 343-346. 



A method is described for determination 

 of phosphorus in quantities from 5-7 ^g. 

 to 2m^g. The sample is digested with 

 H2SO4 in a sealed tube, and the phosphorus 

 is converted to phosphomolybdic acid. The 

 phosphomolybdic acid is extracted with octyl 

 alcohol, reduced with stannous chloride, 

 and measured spectrophotometrically . 



Octyl alcohol extracts phosphomolybdic 

 acid, but not appreciable amounts of molyb- 

 dic acid; an improvement over the isobutyl 

 alcohol used by Berenblum and Chain. 



Schlenk, H . and R . T . Holman 



1950. Separation and stabilization of 

 . fatty acids by urea complexes. Jour- 



^ nal of the American Chemical Society, 



• 72: 5001-5004. 



The use of urea complexes for separation 

 of fatty acids is described, and factors in- 

 fluencing the separation are discussed. 



Urea complexes of unsaturated fatty acids 

 are not subject to autooxidation. 



Schlenk, H., J. L. Gellerman, J. A. Tillotson, 

 and H . K . Mangold 



1957. Paper chromatography of lipides. 

 . Journal of the American Oil Chemists 



2, Society, 34: 377-386. Chemical Ab- 



stracts, 51:15689c (1957). 

 Detailed procedures are given for the 

 qualitative and quantitative paper chroma- 

 tography of lipids, and applications of the 

 methods. Rf values for 16 fatty acids are 

 given. 



Schmidt, C, J. Benotti, B. Herschman, and 

 S.J. Thannhauser 



1946. A micromethod for the quantitative 

 partition of phospholipid mixtures into 

 •^ monoaminophosphatides and sphingo- 



▲ myelin. Journal of Biological Chemis- 



try, 166 : 505-511. 

 The monoaminophosphatides are removed 

 by selective saponification with KOH, and 

 the value of sphingomyelin phosphorus is 

 calculated as the difference between phos- 

 phorus values of total phospholipid and sa- 

 ponified phospholipid. 



Schmidt, G., L. Hecht, P. Fallot, L. Green- 

 baum, andS. J. Thannhauser 



1952. The amounts of glycerylphosphoryl- 

 choline in some mammalian tissues. 

 A Journal of Biological Chemistry , 197 : 



601-609. 

 Glycerylphosphorylcholine was determined 

 as the difference in the amount of choline 

 reineckate obtained before and after 20 min- 

 ute hydrolysis of aqueous tissue extracts 

 with 10 N HCl. Recovery of added glyceryl- 

 phosphorylcholine was quantitative within 

 limits of error of the method (+ 5%) . 



Schmidt, G., B. Ottenstein, W. A. Spencer, 

 C. Hackethal, andS. J. Thannhauser 



1957. Quantitative partition of acetal 

 phospholipides and of free lipide alde- 



A hydes. Federation Proceedings , 16: 



832-835. ~ 



The lipid aldehydes are converted to 

 Schiff-negative compounds by incubation for 

 16 hours in an aqueous emulsion at pH 8.3 

 and room temperature. The acetal phospho- 

 lipids are then determined colorimetrically 

 using Schiff's reagent. 



Schmidt, G. 



1958. Quantitative estimation of fatty 

 acids on filter paper . Naturwissen- 



# schaften, 45: 41. Chemical Abstracts, 



52: 885 Id (1958). 

 Mercuric acetate esters of the fatty acids 

 are chromatographed on filter paper and 

 located by conversion of the acetate to sul- 

 fide. 



61 



