Schmidt, L. H. 



1935 . The nature of the difference in 

 phospholipid content of oxalated and 

 A heparinized plasma. Journal of Bio- 



logical Chemistry , 109 : 449-453. 

 The higher phospholipid content of 

 heparinized plasma than of oxalated plasma 

 is due to shrinkage of red blood cells and 

 consequent increase in plasma volume of 

 the oxalated blood. 



Schmidt-Neilson, K. 



1942. Microtitration of fats in amounts 

 of 10" gram. Compte rendu des 

 _ travaux du laboratoire Carlsberg, 



* Serie chimique, 24: 233-246. Chem - 

 ical Abstracts, 38:2225^(1944). 



The sample is saponified, and the fatty 

 acids are freed, extracted with toluene, and 

 titrated with tetramethylammonium hydrox- 

 ide. 



Schmidt-Neilson, K. 



1944. Microdetermination of the iodine 

 number of fat in quantities of 10"^ gram. 

 Compte rendu des travaux du labora- 

 % toire Carlsberg, Serie chimique , 25: 



87-96. Chemical Abstracts, 40:20^3 

 (1946). 

 The I number is determined by saturating 

 the double bonds with Br .and titrating ex- 

 cess Br iodometrically. 



Schmidt-Neilson, K. 



1944. Extraction and fractionation of 



the lipides in one milligram of tissue. 



Compte rendu des travaux du labora- 

 ^ toire Carlsberg, Serie chimique , 25 : 



• 97-105. Chemical Abstracts, 40:21737 

 (1946). 



Frozen tissue is sliced and the tissue is 

 destroyed with KOH. Unsaponifiable lipids 

 are extracted with toluene, the fatty acids 

 are freed from their soaps by adding HCl, 

 and the freed fatty acids are extracted with 

 tolutne . 



Schon, H. and F. Gey 



1956. Elution chromatographic separa- 

 . tion of free and esterified cholesterols 



in organ and serum fats and direct col- 

 orimetric determination. Zeitschrift 



flir physiologische Chemie, 303 : 81- 

 90. Chemical Abstracts, 50:"l693 4f 

 (1956). 

 Free and ester cholesterol were separated 

 from fat by elution from an AI2O3 column 

 with petroleum ether-ethanol mixtures. The 

 cholesterol was estimated by the Tschugaeff 

 reaction . 



Schoenheimer, R . and W . M . Sperry 



1934. A micromethod for the determina- 

 tion of free and combined cholesterol 

 ■ (cholesterol in blood). Journal of Bio - 



logical Chemistry , 106: 745-760. 

 Free cholesterol is precipitated as the 

 digitonide, and total cholesterol is precip- 

 itated as the digitonide after hydrolysis of 

 the sample. The color is developed with 

 acetic acid, acetic anhydride, and sulfuric 

 acid, and read at 610-620 mp. . The method 

 requires 0.2 cc. of serum or blood. Stand- 

 ard deviation for free cholesterol is 1.48%; 

 for total cholesterol is 1. 16%. 



See Sobel and Meyer (Journal of Biological 

 Chemistry, 157: 255, 1955) for a modifica- 

 tion for use with free cholesterol. 



Schuette, H. A. andS. Dal Nogare 



1951. An oxidation-adsorption method 

 for analysis of methyl ester fractions . 

 ■^ Journal of the American Oil Chemists 



# Society, 28: 229-231. Chemical Ab- 

 stracts. 45:6402g(1951). 



A method is described for the separation 

 of saturated from unsaturated methyl esters 

 of the fatty acids. The unsaturated compo- 

 nents are oxidized with permanganate, and 

 the oxidation products are adsorbed on a 

 column of alumina which has been treated 

 with bromothymol blue . 



Schwarz, H. P.,, L. Dreisbach, R. Childs, and 

 S. V. Mastrangelo 



1957. Infrared studies on tissue lipides. 

 Annals of the New York Academy of 



* Sciences. 69: 116-130. 



Tissue lipids were chromatographed on 

 silicic acid with MeOH and CHCl3-MeOH 

 as eluting solvents, and the eluted fractions 

 were analyzed by infrared spectrophoto- 

 metry. 



62 



