Sperry, W . M . and F . C . Brand 



1955. The determination of total lipides 

 in blood serum . Journal of Biological 



• Chemistry , 213 : 69-76. 



Lipids are determined gravimetrically 

 after CHCl3-MeOH extraction and purifica- 

 tion. The use of N2 atmosphere helps to 

 overcome partial insolubility of the lipid 

 residue. Sperry' s ( Journal of Biological 

 Chemistry, 209: 377, 1954) and Folch's 

 ( Federation Proceedings , 13: 209, 1954) 

 purification procedures remove all urea 

 and presumably all other water-soluble 

 contaminants . 



Sperry, W. M. 



1955. Lipide analysis. In Methods of 

 Biochemical Analysis (D. Click, Ed- 



# itor). New York, Interscience Publ. 

 Inc., Vol. U, pp. 83-111. 



A description and discussion of methods 

 of lipid analysis suitable for use with blood 

 tissues. 



Spiteri, J . and G . Nunez 



1952. Partition chromatography of fat- 

 . soluble substances. Compte rendu , 



* 234: 2603-2604. Chemical Abstracts, 



• 47:941f (1953). 



A method is described for the separation 

 of higher fatty acids, alcohols, sterols, 

 vitamins, etc. from a mixture by chroma- 

 tography on triglyceride -impregnated fil- 

 ter paper. 



Spiteri, J. 



1954. Partition chromatography of fat 



. acids. Bulletin de la Societe de chimie 



* biologique , 33: 1355-1362. Chemical 



• Abstracts, 49: 1325f (1955). 



Good separations of higher fatty acids 

 were obtained by chromatography on paraf- 

 fin -impregnated filter paper. 



Stahli, H. 



1955. Determination of the iodine num- 

 ber. Mitteilungen aus dem Gebeit der 

 Lebensmitteluntersuchung und Hygiene, 

 46: 121-162. Chemical Abstracts, 49: 

 14345b (1955). 



A comparison of the Hanus, Kaufmann, 

 and Wijs methods. A modification of the 



Wijs solution is presented. 



Stern, I. andB. Shapiro 



1953 . A rapid and simple method for the 

 determination of esterified fatty acids 



# and for total fatty acids in blood. Jour- 

 nal of Clinical Pathology, 6: 158-160. 



A method is described for determination 

 of fatty acids by hydroxamic acid formation 

 and colorimetric measurement of the color 

 produced with FeCl3 . Standard deviation 

 + 4%. 



Stewart, C. P. and E . B. Hendry 



1935. The phospholipins of blood. Bio- 



A chemical Journal, 29: 1683-1689. 



The Fiske-Subbarow method for deter- 

 mination of phosphorus is discussed. Con- 

 ditions for accurate use of the F-S method 

 are described. 



Stoffel, W., F. Chu, and E . H. Ahrens, Jr. 

 1959. Analysis of long-chain fatty acids 

 j^ by gas-liquid chromatography. Micro- 



^ method for preparation of methyl esters. 



• Analytical Chemistry , 31: 307-308. 



A method is described for preparation of 

 methyl esters of long-chain fatty acids ob- 

 tained from biological material. 1 to 10 mg. 

 of the lipid material is dissolved in a meth- 

 anol-5% HCl-benzene mixture and inter- 

 esterified by refluxing at 80-100° for 2 

 hours. The methyl esters are isolated 

 from the reaction mixture by microsubli- 

 mation and are then ready for separation 

 by gas -liquid chromatography. Recovery 

 of esters is over 95% of theory. 



Sturges, S. and A. Knudson 



1938. Application of the Schoenheimer- 

 Sperry method to the determination of 

 cholesterol and cholesterol esters in 

 tissues. Journal of Biological Chemis- 

 try, 126: 543-550. 

 A microcolorimetrlc method for deter- 

 mination of tissue cholesterol which is based 

 on the Schoenheimer -Sperry method ( Journal 

 of Biological Chemistry, 106 : 745, 1934) is 

 described. 



Surrey, B. D. 



1954. Modified iodometric determination 



66 



