of organic peroxides. Analyst, 79 : 



86-90. 

 The method is similar to that of Lea 

 (Journal of the Society of Chemical Indus - 

 try, ^: 106, 1945). The reagents are all 

 mixed in a boiling solution of AcOH and 

 CHCI3, which makes deaeration of the re- 

 agents and use of an inert atmosphere un- 

 necessary. 



Svennerholm, L . 



1954. Partition chromatography of brain 

 , gangliosides on cellulose. Acta chem- 



ica Scandinavia, 8: 1108. 

 Gangliosides were separated from the 

 lipid extract of gray matter of human brain 

 by chromatography on cellulose. 



Swahn, B. 



1952. A method for the localization and 

 determination of serum lipids after 



■^ electrophoretic separation on paper. 



9 Scandinavian Journal of Clinical and 



Laboratory Investigation, 4: 98-103. 

 Lipid components of serum are separated 

 by paper electrophoresis and treated with a 

 50% alcoholic solution of Sudan Black . The 

 color absorbed by the lipids may be meas- 

 ured by direct colorimetry on the paper, or 

 by elution of the spots and reading in a col- 

 orimeter . 



Swahn, B. 



1953. Studies on blood lipids: I. A micro- 

 method for determination of total lipids 

 in serum. II. A micromethod for deter- 

 mination of serum lipids after electro - 



^ phoretical separation on filter paper . 



III. Electrophoretical mobility of "chy- 

 lomicrons" . Scandinavian Journal of 

 Clinical and Laboratory Investigation, 

 5 (Suppl . 9): 1 14 pp . 

 A method for determination of total lipids 

 in 0.02 ml. of serum is described. The se- 

 rum is placed on a filter paper and dried, 

 and the paper is placed in an ethanolic solu- 

 tion of Sudan Black B. After washing and 

 drying, the dissolved dye is extracted and 

 its concentration is measured colorimetri- 

 cally. Effects of variables are discussed. 

 Error is 1.89%. 



See also: Bernes and McDonald (Archives 



of Biochemistry and Biophysics, 70: 49, 

 1957), who separated Sudan Black into 10 

 components by chromatography. All frac- 

 tions were found to stain lipids, but to vary- 

 ing degrees . 



Swem, D., H. B. Knight, O. D. Shreve, and 

 M. R. Heether 



1950. Comparison of infrared spectro- 

 photometric and lead salt alcohol meth- 

 ods for determination of trans -octa- 

 _ decenoic acids and esters. Journal of 



the American Oil Chemists Society, 27: 

 17-21. Chemical Abstracts, 44:2260f~ 

 (1950). 

 The infrared method was found to be more 

 accurate, specific, and rapid than the lead 

 salt method. 



Szent-Gyorgi, A. 



1957. Detection of chromatographic spots 

 on paper . Science, 126 : 75 1 . 

 The developed chromatogram is cooled in 

 liquid nitrogen and viewed under ultra violet 

 light. Spots may be detected by their phos- 

 phorescence. 



Taurog, A., C. Enteiunan, B. A. Fries, and 

 I. L. Chaikoff 



1944. An adsorption procedure for the 

 separation of choline -containing from 

 "^ non -choline -containing phospholipids 



A of liver. Journal of Biological Chemis- 



try, 155 : 19-25. 

 Total phospholipids are adsorbed on MgO 

 and the choline -containing phospholipids are 

 eluted with MeOH. 



Taylor, W . E . and J . M . McKibbin 



1953. The determination of lipide inosi- 

 tol in animal tissues. Journal of Bio- 

 logical Chemistry , 201: 609-613. 

 A method is described for determination 

 of inositol in tissues by microbiological 

 assay with Saccharomyces carlsbergensis 

 on the acid hydrolysates of purified lipid 

 extracts and turbidimetric measurement of 

 the yeast growth. 



It was inconvenient to use Saccharomyces 

 cerviseae as the test organism as its me- 

 dium turns very dark after autoclaving, mak- 

 ing direct turbidimetric readings unsuitable . 



67 



