C.2-C24 ^^^^y acids were chromatograph- 

 ed on a dichlorodimethylsilane-treated col- 

 umn of silicic acid by using 2,2,4-trimethyl- 

 pentane as stationary phase and eluting with 

 aqueous methanol . The eluted acids are 

 measured by titration. Error is less than 



2.5%- 



An apparatus for automatically and con- 

 tinuously changing the composition of the 

 eluting solvent and a semiautomatic, motor 

 driven microburette are described. 



Van Handel, E . 



1959. Separation and chemical assay of 



# lipide classes . Journal of the Ameri- 

 A can Oil Chemists Society, 36: 294-297. 

 ■ Chemical Abstracts, 53:17226h (1959). 



A review and discussion of methods for 

 microdetermination of cholesterol, triglyc- 

 erides, and phospholipids . 



Van Handel, E. and D. B. Zilversmit 



1953. Micromethod for the direct deter- 

 mination of serum triglycerides . Jour - 



# nal of Laboratory and Clinical Medicine , 

 50: 152-157. 



Triglycerides are determined as glycerol 

 by the chromotropic acid method of Lambert 

 and Neish ( Canadian Journal of Research, 

 28: 83, 1950) after quantitative removal of 

 phosphatides and saponification. 



Wachsmuth, H. and L. van Koeckhoven 



1959. The determination of choline in 

 blood serum . Clinica Chimica Acta, 

 4: 206-212 (In French). Chemical Ab- 

 stracts, 53:12381g(1959). 

 Precipitation of choUne by silico-tungstic 

 acid and reduction of the silico-tungstate 

 with HSO3" gives a color reaction which is 

 600 times more intense than the choline 

 reineckat^ reaction. 



Weigensberg, B. I. andG. McMillan 



1959. Ultraviolet spectrophotometric 



method for the determination of choles- 

 ■ terol . American Journal of Clinical 



Pathology, 31: 16-25. 



Serum or tissue cholesterol or the cho- 

 lesterol digitonide is determined by spectro- 

 photometric measurement of the absorption 

 of UV lig^t at 204-206 mjx . The method is 

 simple and reproducible, and is 3 to 4 times 

 more sensitive than the Schoenheimer- 

 Sperry method. 



Weil, L. andM. A. Russell 



1942. Studies on plasma phosphatase 

 activity and on blood phospholipids in 

 ▲ rats with obstructive jaundice . Journal 



of Biological Chemistry , 114 : 307-314. 

 A method is described for the determina- 

 tion of the phospholipids in an alcohol -ether 

 extract of 8 mm? of plasma or 4 mm? of 

 blood by a micro modification of the method 

 of Boyd (Journal of Biological Chemistry , 144 : 

 223, 1936). The phospholipids are digested 

 with HCIO4 and color is developed with am- 

 monium molybdate, using aminonaphtholsul- 

 fonic acid as reducing agent. 



Weiss, B 

 1957 



• 



▲ 



The separation of sphingolipides 

 by adsorption chromatography. Jour- 

 nal of Biological Chemistry , 223 : 523- 

 534. 

 A method is described for chromatograph- 

 ic separation of sphingolipides of the central 

 nervous system by gradient elution with chlo- 

 roform and methanol from a silicic acid col- 

 umn. 



Attempts to use the method of Taurog, et 

 al, (Journal of Biological Chemistry, 155: 

 19, 1944) for separation of choline -contain- 

 ing from non-choline-containing phospho- 

 lipids on magnesium oxide were unsuccess- 

 ful. 



Diminished resolving power was observed 

 when Hyflo Super -Cel was added to the ad- 

 sorbent, or when the silicic acid was added 

 to the column as a chloroform suspension 

 rather than a chloroform -methanol suspen- 

 sion. 



72 



