determined spectrophotometrically at 327 

 m/i. Accuracy is + 5% with 50-400 /i-g. of 

 choline hydrochloride. 



Wittenberg, J, B. 



1955. The separation of sphingosine and 

 related compounds by reversed phase 



-^ partition chromatography. Journal of 



Biological Chemistry, 216 : 379-390. 



After conversion to their N-succinyl de- 

 rivatives with succinic anhydride, sphingo- 

 sine and related compounds are separated 

 on a dichlorodimethylsilane -treated column 

 of diatomaceous earth. 



A method for assay of sphingosine com- 

 pounds is proposed which entails conversion 

 of sphingosine and related compounds to 

 their N-succinyl derivatives and titration 

 with alkali of the carboxylic acid group 

 which has been introduced. Three to 100 

 /imoles were determined with an accuracy 

 of +5%. 



Wittenberg, J. B., S. R. Korey, and F. H. 

 Svenson 



1956. The determination of higher fatty 

 aldehydes in tissues. Journal of Bio- 

 logical Chemistry , 219 : 39-47. 



A method is described for the estimation 

 of higher fatty aldehydes in tissue lipids by 

 colorimetric measurement of their p-nitro- 

 phenylhydrazone derivatives. The method 

 is specific for higher aldehydes, and lower 

 aldehydes and keto acids do not interfere. 

 Determines 0. 1 to 7 umoles within 5%. 



Wittenberg, J. B. 



1957. The separation of the C5-C,2 

 fatty acids by reversed-phase partition 



^ chromatography . Biochemical Journal, 



• 65: 42-45. 



Chloroform -Skellysolve S-water-methanol 

 solvent systems were used to separate the 

 C^-C j2 fatty acids on a dichlorodimethyl- 

 silane -treated column of Hyflo Super -Cel. 



Witter, R. F., G. V. Marinetti, A. Morrison, 

 and L. Heicklin 



1957. Paper chromatography of phospho- 

 lipides with solvent mixtures of ketones 

 and acetic acid. Archives of Biochem- 



• 



istry and Biophysics, 68: 15-20. 



Lysolecithin, sphingomyelin, phospha- 

 tidyl ethanolamine, lecithin, andphospha- 

 tidic acid in quantities of 10 to 25 jxg. in 

 20/^1. were separated by paper chromatog- 

 raphy using mixtures of various ketones and 

 acetic acid as solvent systems. 



Wolman, M. 



1950. Staining of lipids by the periodic- 

 acid-Schiff reaction. Proceedings of 

 the Society for Experimental Biology 

 and Medicine, 75: 583-585. 

 Schiff's reagent will stain unsaturated 

 lipids after they have been oxidized with per- 

 iodate. Sphingolipids will stain even if they 

 do not contain a carbohydrate. 



Woolley, D. W. 



1941 . A method for the estimation of ino- 

 sitol . Journal of Biological Chemistry , 

 140 : 453-459. 



A basal medium was developed which sup- 

 ported practically no growth under the ex- 

 perimental conditions. Inositol content of 

 the sample material added to the basal me- 

 dium was determined by colorimetric meas- 

 urement of the turbidity produced by the 

 yeast Saccaromyces cereviseae. 



It was noted that inositol does not produce 

 marked stimulation unless the basal medium 

 was otherwise complete for optimal growth. 



Attempts to use Eastcott's basal medium 

 (Journal of Physical Chemistry , 32: 1096, 

 1928) were not entirely successful. 



WooUey, D. W. 



1943 . Isolation and partial determination 

 of structure of soy bean lipositol, a new 

 ■^ inositol -containing phospholipid. Jour- 



▲ nal of Biological Chemistry , 147: 581- 



591. 

 Lipositol was prepared by extraction with 

 CHCI3 and recrystallization from CHCI3 

 with methanol and ethanol . 



Wren, J. J. and H. K. Mitchell 



1958. Silicic acid chromatography of 

 j^ lipids of whole human blood. Proceed - 



^ ings of the Society for Experimental 



* Biology and Medicine, 99: 431-435. 



Blood lipids were separated into 20 com- 

 ponents by chromatography on silicic acid. 



74 



