Chemistry, 27: 764-768. 

 All of the individual saturated fatty acids 

 C2-C14, the C|3-Ci5 fatty acids differing 

 by two C in chain length, and the dicarbox- 

 ylic acids C2 to C22 were separated on a 

 silicic acid column. Methyl cellosolve- 

 water was used as a stationary phase, and 

 Skellysolve B, n-butyl ether, or their mix- 

 tures were used as eluting solvents. 



Zilch, K . T . and H.J. Dutton 



1951. Analysis of fat oxidation product 

 ■^ by countercurrent distribution methods. 



Analytical Chemistry, 23: 775-778. 

 Effects of various functional groups on 

 the distribution of compounds were studied. 

 Partition coefficients are given for a num- 

 ber of model compounds . 



Zilversmit, D. B. and A. K. Davis 



1950. Micro -determination of plasma 

 phospholipids by trichloracetic acid 

 A precipitation. Journal of Laboratory 



and Clinical Medicine, 35: 155-160. 

 A method is described for determination 

 of phospholipids in 0.2 ml. of plasma. The 

 phospholipids are precipitated with trichlo- 

 racetic acid and phosphorus is determined 

 colorimetrically after perchloric acid di- 

 gestion of the precipitate. 



Zlatkis, A., B. Zak, and J. Boyle 



1953. A new method for the direct deter- 

 mination of serum cholesterol. Jour- 

 ■ nal of Laboratory and Clinical Medicine , 



41: 486-492. 

 A sulfuric acid, acetic acid, and ferric 

 chloride reagent is added directly to a 0. 1 

 ml. sample of serum and the color is read 

 spectrophotometrically at 400-700 m/.i. 

 Cholesterol and cholesterol esters yield the 

 same molar color, thus eliminating the need 

 for saponification . The method compares 

 well with other methods, and is rapid and 

 reproducible. 



Zuckerman, J. L. andS. Natelson 



1948. A convenient and rapid procedure 

 for total cholesterol estimation using 

 an acid chloroform extraction. Journal 

 of Laboratory and Clinical Medicine, 

 33: 1322-1325. 

 Sulfuric acid and chloroform are added to 

 the serum sample and mixed. The sample 

 is centrifuged and the acid and precipitated 

 protein are removed. Acetic anhydride and 

 sulfuric acid are added to an aliquot of the 

 chloroform solution and the color is read at 

 625 m^^. The method requires about 45 min- 

 utes . Results are comparable to those ob- 

 tained by Bloor's extraction method. 



76 



INT.DUP.,D.C.61- 97961 



