261 



The fully developed trophozoite (scliizontj bends on itself, becoming 

 U-shaped (figs. 9 and 10), until its ends appose (fig. 11) and fuse. The 

 nucleus of such a ring shaped schizont, at first lying chiefly at one side, 

 begins to break up, usually into unequal portions (figs. 12 and 14\ 

 especially as seen in specimens stained by the Romanowski method. 

 There are often eight or nine such parts or dots seen (fig. 13). 

 Around each nuclear portion some refractile protoplasm of the parasite 

 aggregates, and so '^rosettes-stages are formed (figs. 15 and 16), consist- 

 ing of merozoites in contact forming a spheroidal mass. The diameter 

 of such a schizont, dividing into merozoites, is from 2,5 to 3,5 /.i 

 approximately. The latter, when fully formed, separate. Curious arrange- 

 ments of partially separated merozoites have been seen (fig. 17). 



Regarding free stages of this parasite in the blood-plasma of the 

 host, I am of opinion that such occur in my preparations, but extreme 

 caution needs to be exercised in making definite statements on such 

 matters. 



I have found parasites in the spleen-pulp of infected newts, and in 

 some cases, especially well marked in one, the spleen appeared enlarged. 



As to the sporogony of this haemogregarine unfortunately I have 

 as yet no observations. In view of Siegel' s recent paper on the sporo- 

 gony of iïaewo^/*e^arma sfe^«;^o^'i [13], and Schaudinn's remark on 

 that of Karyolysus lacertarum [12] aj^pended as a footnote to Si egei' s 

 paper, it appears highly probable that the sporogony of this haemo- 

 gregarine of the newt occurs in an intermediate host, a blood-sucking 

 organism, perhaps a leech. The idea that the sj)orogony of Laiikesterella 

 raiiarum [L. miniina of Hint ze) takes place in the intestinal epithelium 

 of the same host [4], is apparently incorrect. A parasite from the in- 

 testinal epithelium of the newt was described by Steinhaus as long 

 ago as 1891, under the name oi Karyophagus {Cytophagus) tritonis [14]. 

 In Wasieliewski's "Sporozoenkunde" [15] this is placed under the 

 AcystosporicUa. Minchin [10, p. 270] states that this is the Eimerian 

 stage of a Coccidian, and Schaudinn suggests that the parasites in the 

 intestinal cysts of the frog mentioned by Hintze were Coccidian or 

 some such form [12]. The intestinal tracts of many of the infected newts 

 have been preserved and an examination of the same is in progress. In view 

 of Schaudinn's and Siegel' s researches I hardly expect to obtain a 

 connection between the lif e-liistories of a Karyophagus-\ike form and the 

 haemogregarine described in this paper. 



As to the systematic position of this parasite I would place 

 it among the haemogregarines, as its trophozoite is vermiform and endo- 

 globular in the early stages. From its size the genus Fi)'oplas))ia is 

 perhaps suggested, but the trophozoites are vermiform, and a rosette 



