20 



After quoting several portions of this report at some lengtii, he 

 criticises the views therein expressed. He doubts the possibihty 

 of a large area of sparsely populated bush country, thick with game 

 and G. morsitans, being free from trypanosomes. He considers it 

 probable that the "pathogenic trypanosomes of cattle and domestic 

 animals have been derived from those of wild game in which they are 

 innocuous. 



That disease or death in cattle in the area visited is invariably due 

 to trypanosomes is inadmissible, as several other diseases are known 

 to have occurred among them. The identity of these and the native 

 names for them are discussed. The supposition that more or less 

 speedy death follows infection with trypanosomes is not borne out by 

 facts, as animals infected in June and July 1913 were still alive and in 

 excellent condition early in 1914, nor is the conclusion justifiable that 

 an animal has been recently infected because of the occurrence of 

 T. vivax or T. nanuni in the peripheral blood. 



Some account is given from the evidence of natives of earlier out- 

 breaks of trypanosomiasis in this part of Uganda and of the movements 

 of cattle that have contributed to the present distribution of the 

 disease. With regard to the movements of fly, experunents showed that, 

 whereas a slow pedestrian will soon throw off " following fly," the pace 

 of a cyclist seems to exert an irresistible attraction, and large numbers 

 will follow for miles ; the author therefore considers it possible that 

 the introduction of bicycles among the natives of Busindi, dating from 

 1908, has had a definite influence on the recent epidemic. 



A number of experunents in the belt of G. morsitans at Masindi are 

 described. Wild flies caught at various places in the belt were fed 

 upon suitable anunals, such as monlceys, and gave rise to infections of 

 T. brucei, T. pecorum, T. nanum, T. vivax, and T. unifonne. Two 

 cases of natural infection by T. brucei and T. pecorum in dogs are 

 recorded. Dissection of the flies used showed that infections of the 

 proboscis only is ascribable to T. vivax or T. unifonne ; those of the 

 proboscis and gut to T. nanum or T. pecorum ; those of the gut and 

 salivary glands to trypanosomes of the T. brucei group ; and those of the 

 gut only to immature infection by T. nanum, T. pecorum or T. brucei. 

 As evidence of the presence of T. brucei in G. morsitans in the 

 Masindi belt before Miss Robertson's arrival, it is stated that the 

 native dogs were dying in 1912-13 with symptoms of keratitis and that 

 this symptom is almost always due to T. brucei, and rarely if ever to 

 T. pecorum. 



In another series of experiments, wherever a positive salivary gland 

 was found, the animal fed upon developed T. brucei, and T. nanum 

 appeared to be more common in the wild flies than T. pecorum. Of 

 inoculations made from the blood of 30 head of game, 16-6 per cent, 

 gave positive results, 6-6 per cent, being due to T. briicei. Similar 

 experiments were performed in other tsetse districts of the Northern 

 Province to determine the distribution of the brucei-\\ke trypanosome. 

 In the Ngussi River district, G. p)aUidip)es, G. palpalis and G. fusca 

 occurred. Of a total of 165 flies dissected, gut and salivary gland 

 infection was found in 3 per cent, of G. pallidipes only, and T. brucei 

 was obtained in feeding experiments. On the Sonya Peninsula and 

 the shore of Lake Albert, G. palpalis only was found ; among 407 flies 

 dissected, no salivarv gland infections were observed. Of 20 head of 



