134 



Dissection showed infected specimens early in the investigation, 

 namely, an example of A. quadrimaadatys on 21st September (collected 

 18th September) and another of A. pmictipennis on 29th September 

 (collected 26th September). 



An effort was made in the region of Alabama to determine the 

 season when malaria ceased to exist in a transmissible form. One 

 infection was noted in an A. ^ quadrimaculatiis examined on 15th 

 November in the shape of a single oocyst apparently of stunted develop- 

 ment and similar in character to those seen in mosqiiitos kept in the 

 laboratory at low temperatures for two months or longer, and which 

 were not observed to develop to maturity during this period. 



Le Prince (J. A. A.) & Griffitts (T. H. D.). Flight of Mosquitoes. 

 Studies on the Distance of Flight of Anopheles quadrimaculatus. — 

 TJ.S. Public Health Repts., Washington, xxxii, no. 18, 4th May 

 1917, pp. 656-659, 3 figs. 



In the Southern United States Ano])heles quadmnaculatus is probably 

 the principal carrier of malaria and these studies of flight-distance, made 

 in 1916 inS. Carolina, have been devoted to this species, A. pimctipennis 

 and ^. crucians not having been studied from this point of view as yet. 

 The experiments were planned on the same lines as those carried out 

 with the flight of a Panama species, A. tarsimacidatus, at Gatun. At 

 Stevens Creek large numbers of A. quadrimacidatus were captured, 

 either at their breeding places or in heavily infested houses. They 

 were stained by spraying with a 1 per cent, aqueous solution of eosin 

 from an atomizer and liberated from a point in the breeding area 

 selected as a liberation station. On the first day only a few were set 

 free, but on the second day a large number were liberated, all at one 

 point. For ten days following the liberation of the first batch daily 

 catches, totalling 1,542, were made in inhabited houses, barns and 

 stables within 1| miles from the point of liberation. The first stained 

 specimen was recovered on the third day at a distance of 5,565 feet. 

 On the following day a second specimen was caught at 2,800 feet. 

 Two others were captured on the sixth day at 3,245 feet. All these 

 stained specimens were engorged, and were taken during the day- 

 time, apparently near where they fed. At Fort Lawn it was desired 

 to determine if A. quadrimaculatus would cross the river there. The 

 liberation station was on the east bank of the Catawba where there 

 were only one or two houses within one mile. On the west shore 

 houses were more numerous and there were cattle in the fields — 

 a plentiful blood supply. About 270 A. quadrimaculatus and 30 

 A. punctipennis were captured in houses and barns within one-half mile 

 of the Catawba on the west side. After staining they were liberated 

 from the east side. Within 72 hours two of the marked A. quadri- 

 maculatus were found in a negro shack on the west side. On the 

 following day a third A. quadrimaculatus was taken at the same place. 

 It is worthy of note that a large proportion of the Anophelines 

 originally captured for the experiment came from this cabin. The 

 flight distance was 3,090 feet from the point of liberation (providing 

 the flight was in a direct line), 800 feet being over the waters of the 

 Catawba River. An examination of many houses from three-quarters 

 of a mile to a mile distant from the river proved negative. 



