440 



Reixkixg (O. A). Notes on Coccids and Aleyrodes on various Hosts in 



Indo-China and Siam. — Philippine Aqyiciillufisi, Los Banos, 

 ix, no. 6-7, January-February 1921, p. 185. [Received 11th 

 July 1921.] 



In a collection of Coccids and Alcurodids made in June and July 1920 

 the following were obtained in Indo-China : — Coccus viridis, Green, on 

 coffee {Coffca liber ica) ; Chrysomphalus aurantii, Mask., Lepidosaphes 

 beckii, Newm., Paralccanittm expansion var. quadratum. Green (?) 

 and Phenacaspis eitgeniae, Mask. (?) on black pepper {Piper nigrum) ; 

 Paralecaniiim expansum var. quadratum, Green (?) on mango {Mangifer'a 

 indica) ; and Coccus sp. on Gardenia sp. 



In Siam, Saissetia nigra, Nietn., was found on Hibiscus sp., and a 

 species of Trialeurodes on castor oil plant {Ricinus communis). 



ZoTTA (G.). Sur la Transmission exp^rimentale du Leptomonas 

 pvrrhocoris, Z., chez des Insectes divers. — C. R. Soc. Biol., Paris, 

 Ixxxv, no. 23, 25th June 1921, pp. 135-lvS7. 



This paper records the results of experiments in the direct inoculation 

 of Leptomonas pyrrhocoris into the general cavity of Galleria mello- 

 nella (caterpillar), Carausius morosus, Calliphora sp., and Tenebrio 

 molitor (larvae), which have no fiagellosis of their own, and Notonecta 

 glauca and Naucoris cimicoides, which have a special Leptomonad 

 intestinal parasite. 



L. pyrrlwcoris, the normal parasite of Pyrrhocoris apterus, can be 

 sucessfuUy inoculated into N. glauca, N. cimicoides, G. mellonella 

 (caterpillar) and Calliphora sp. In these the flagellates find an 

 excellent developmental medium and — especially in the larvae of 

 T. molitor and G. mellonella — multiph^ greatly after 24-36 hours. 

 After a few passages their \'irulence is so much increased that a small 

 quantity of infected blood suffices to reproduce the infection. The 

 larvae of T. molitor, and particularly those of G. mellonella, can resist 

 the infection for a somewhat long period, and by regular passages 

 it is possible to maintain the flagellates indefinitely in these insects. 

 The caterpillar of G. mellonella is an excellent laboratory reservoir 

 of the virus. 



Infection was not achieved with the adult of Hvdrophilus piceus, 

 and a Phasmid, Carausius morosus, was also refractory, though the 

 resistance of the latter does not seem to be absolute and may break 

 down with a different technique. 



In insects liable to infection there occurs an intensive phagocytosis, 

 which is, however, unable to check the course of the infection. In 

 C. morosus, in which the infection does not succeed with certainty, 

 progressive degeneration of the flagellates free in the plasma 

 points to a marked humoral reaction side by side with the intense 

 phagocytosis. 



The infection is usually fatal in the caterpillar of G. mellonella, and 

 though pupation often occurs, the pupae die. In T. molitor the infec- 

 tion persists through all stages, and the imagines alwa^'s harbour 

 Leptomonads in their body fluid. 



In order to speak of a true and definitive adaptation of L. pyrrhocoris 

 to the insects mentioned above it is necessary to achieve an infection 

 per OS as well. A paper on this subject will be published later. 



