Some specimens which were mounted directly in cold glycerine 

 appeared to be very satisfactory at first, but, after standing for 

 some weeks, masses of needle-shaped crystals formed over the 

 exterior of the insect, so as to obscure its outlines and render the 

 mount practically useless. No analysis was made of these crys- 

 tals, but they may have been phosphates which had been dissolved 

 in the internal juices of the body. The glycerine gradually ex- 

 tracts these juices, and, as the phosphates held by them would be 

 insoluble in glycerine, they immediately formed a crystalline pre- 

 cipitate. 



Laboratory results have been verified as far as possible by field 

 observation. 



Egg. (Plate IV., Fig. 29.) 



The females deposit their eggs in the tissue of the leaf, selecting 

 those parts which are fresh and tender, and appear to oviposit as 

 readily during the night as they do in daylight. The process of 

 oviposition is as follows : The female arches her abdomen so as to 

 bring the weight of her body to bear upon the slender four-valved 

 ovipositor (Plate III., fig. 18), which is attached to the ventral 

 side of the eighth and ninth segments (Plate IV., fig. 31). The 

 ovipositor is then slowly worked down through the epidermis of the 

 leaf and into its cell structure, the abdomen being gradually lowered 

 during this operation, though otherwise the insect appears to be 

 perfectly quiet. Then, by successive contractions of the abdomen, 

 the egg is pushed back under the epidermis of the leaf. The com- 

 plete operation requires about a minute and a half, after which the 

 female usually moves off a short distance and begins feeding. 

 Occasionally the ovipositor becomes so firmly wedged in the leaf 

 as to hold its possessor prisoner for some time, frequently until 

 death results. 



The eggs may be readily separated from the tissue of the leaf 

 and mounted for more careful study with the microscope, but care 

 must be taken not to crush or distort them in these operations. 



The eggs are reniform, and vary in length from .265 mm. to 

 .33 mm., and in width from .085 mm. to .145 mm. The average 

 dimensions, taken from about twenty-five eggs, are : length, .288 

 mm.; width, .11 mm. The color is a translucent white. By 

 transmitted light they are seen to be filled with a mass of yolk 

 globules which vary considerably in size. 



The hibernated females begin egg laying very soon after the 

 weather becomes sufficiently warm to start the grass. Specimens 

 of these females show from one to eight fully developed eggs within 

 the abdominal cavity. They may live and continue egg laying for 



