660 GROWTH OF PHAGE AND LYSIS 



The cell contents are then liberated into solution without deformation of 

 the cell wall. 



3. Lysis from without is caused by adsorption of phage above a threshold 

 value. The cell contents are liberated by a distension and destruction of 

 the cell wall. The adsorbed phage is not retrieved upon lysis. No new 

 phage is formed. 



4. The maximum yield of phage in a lysis from within is equal to the 

 adsorption capacity. 



5. Liberation of phage from a culture in which the bacteria have been 

 singly infected proceeds at a constant rate, after the lapse of a minimum 

 latent period, until all the infected bacteria are lysed. 



6. If the bacteria are originally not highly in excess, this liberation is soon 

 counterbalanced by multiple adsorption of the liberated phage to bacteria 

 that are already infected. This leads to a reduction of the final yield. 



The author wishes to express his appreciation for the hospitality extended 

 to him by the Biology Department of the California Institute of Technology 

 during the tenure of a Fellowship of The Rockefeller Foundation. In 

 particular he wishes to record his indebtedness to Dr. E. L. Ellis for constant 

 help and advice and to Mr. F. Gardner for technical assistance. 



REFERENCES 



1. Ellis, E. L., and Delbruck, M., /. Gen. Physiol, 1939, 22, 365. 



2. Bordet, J., Proc. Roy. Soc. London, Series B, 1931, 107, 398. 



3. Krueger, A. P., and Northrop, J. H., /. Gen. Physiol, 1930, 14, 223. 



4. Northrop, J. H., /. Gen. Physiol, 1937, 21, 335. 



5. Northrop, J. H., /. Gen. Physiol, 1939, 23, 59. 



6. Burnet, F. M., and McKie, M., Australian J. Exp. Biol and Med. Sc, 1929, 6, 277. 



7. Burnet, F. M., and Lush, D., Australian J. Exp. Biol, and Med. Sc, 1936, 14, 27. 



8. Scribner, E. J., and Krueger, A. P., /. Gen. Physiol, 1937, 21, 1. 



9. Krueger, A. P., and Strietmann, W. L., /. Gen. Physiol, 1939, 22, 131. 



10. Delbruck, M., /. Physic. Chem., 1940, 8, 120. 



11. Hershey, A. D., /. Gen. Physiol, 1939, 23, 11. 



12. Bronfenbrenner, J., Muckenfuss, R. S., and Hetler, D. M., Am. J. Path., 1927, 3, 562. 



13. Bayne-Jones, S., and Sandholzer, L. A., /. Exp. Med., 1933, 57, 279. 



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