APPENDIX 

 SALINE MEDIA 



Sea Water 



The composition of sea water in terms of hypothetical combinations of 

 ions appears in the following table. Concentration: chlorinity, 19% ; 

 salinity 34-325% . Depression of freezing point, -1-872°C. Density, 

 1-026 at 17-5°C. 



(From Lyman and Fleming (10).) 



Osmotic pressures of sea-water solutions at different salinities and 

 temperatures are given by Wilson and Arons (23). 



Isosmotic Solutions 



The table on page 677 shows concentrations of salt solutions approxi- 

 mately isosmotic with sea water (salinity, 34-325% ). 



Actual concentrations of hygroscopic salts, CaCl 2 , MgCl 2 , Chloride CI, 

 should be determined by argentometric titration. 



The pHs of these isosmotic solutions differ greatly from sea water, and 

 for particular experiments may need adjustment by addition of appropriate 

 acid or base. 



Over the range 9—2 1 % CI, the ratio of NaCl molality to sea water 

 chlorinity can be expressed as 



R = 0-02782+0-000079 (% CI) 



Solutions of salts isosmotic with sea water in this range are described in 

 the following table — ■ 



m.a.— 22* 675 



