appendix: saline media 



677 



(Based on cryoscopic data and isopiestic measurements) 



Artificial Sea Water 



This may be made up by weighing out the amounts of salts shown in the 

 preceding table, or by using isosmotic solutions in the following propor- 

 tions, if these are at hand. 



(1) For most purposes KBr may be omitted. 



Balanced Salt Solutions suitable for Marine Animals 



The tissues of many lower invertebrates will continue to function norm- 

 ally in sea water, which can be used as a physiological medium. Even in 

 more complex forms, crustaceans and cephalopods, isolated tissues can 

 survive and function in sea water, although the blood differs considerably 

 in composition from the latter. Thus the heart beat of Maia persists in 

 sea water, and giant-axons of squid and cuttlefish transmit normally. A 

 variety of physiological media have been advocated and employed by 

 different investigators. Some of these recipes are entirely empirical; others 

 are based on analyses of the chief constituents of body fluids. Some suit- 

 able tissue media appear in the following tables. See also McClendon (1 1), 

 Young (24), Pantin (14), Prosser (16). 



