222 Caroline McGill. 



5. Germ -cells are distinguished from lilament - cells by their 

 o-ranular cytoplasm and by the presence of double nucleoli and yolk- 

 nuclei. 



6. The double nucleoli in both forms arise by the condensation 

 <v nf the basiphile chromatin-spireme around the oxyphile nucleolus, 



this condensation being- comparable with the synapsis stage in 

 spermatogenesis. 



7. Growth area of Flathemis: 



a) Oxyphile nucleoli form in the basiphile mass and pass out 

 of it one after another throughout the growth period of the cell. 



b) These oxyphile bodies dissolve in the nuclear sap, and there 

 is ground for concluding that this material undergoes a chemical 

 change and is reprecipitated as the chromatin-reticulum. 



c) Many of the nucleoli are much vacuolated, indicating a high 

 metabolic activity. 



d) In many nuclei double nucleoli are not found, but a blending 

 of the two differently staining substances occurs, indicating that 

 there may be such a thing as a transformation of the one substance 

 into the other. 



8. Growth Area in Anar: 



a) A single oxyphile nucleolus is always present and it i)ersists 

 during the entire growth period. 



b) This nucleolus is small and unvacuolated, and does not show 

 evidences of active metabolism. 



c) The basiphile nucleolus breaks away from the oxyphile and 

 unwinds into a course, granular spireme which represents all the 

 chromatin in the cell. 



d) The spireme in later growth gives off chromatic granules 

 which pass out along the linin reticulum to form a dense chromatin 

 net-work in the nucleus. 



9. Yolk-nuclei form as granular masses just outside the nuclear 

 membrane. They later break away from the nucleus and divide 

 into several masses which gradually scatter in the cytoplasm. The 

 granules of the yolk-nuclei take chromatin stains. 



10. The intimate relation between nuclein or chromatin and 

 para-nuclein is sliown. 



11. The present study would seem to afford an explanation of 

 The wellkown observation that in resting nuclei the nucleolus is 

 very large and the chromatin is apparently either absent or present 



