228 Caroline McGill, 



Fig. 9. Cross-section of late germinal area showing double nucleoli, a. 



Pig. 10. New oxyphile body forming in the basiphile nucleolus. 



rig. 11. Several oxyphile bodies in the basiphile nucleolus. 



Pig. 12. Oxyphile body passing out of nucleolus. Yolk-nuclei 

 forming. 



Pig. 13. Two oxyphile bodies have passed out of the nucleolus. 



Pig. 14. Pour oxyphile bodies in the nucleus. The yolk-nucleus 

 beginning to scatter through the cytoplasm. 



Fig. 15—17. Differentiation of the double nucleolus by the method 

 of Obst. 



Fig. 18. Oxyphile bodies forming in the basiphile nucleolus. 



Fig. 19. Oxyphile bodies leaving the nucleolus. 



Plate 14. 



{Plathemis lijdia.) 



Pia. 20 — 27 are stained with iron-haematoxylin. 



Yig. 28 30 show living ova examined in normal salt solution. 



Fig. 20. Oxyphile bodies leaving the nucleolus. 



Pig. 21. Shows the liquid state of the oxyphile nucleolus. 



Pig. 22. Very active nucleolus showing large vacuole with chromatm 

 reticulum inside. 



Pig. 23. Oxyphile granule forming inside vacuole of basiphile body. 



Fig. 24. Scattering of yolk granules in cytoplasm. 



Pig. 25. Eapid formation of oxyphile nucleoli. 



Fig. 26. High metabolic activity of nucleolus shown by the presence 

 of vacuoles. 



Pig. 27. The oxyphile nucleolus (r) giving off strings of chromatin 

 granules which go to form the chromatic reticulum. A second oxyphile 

 body (6) is passing out of the basiphile nucleolus («). 



"Fig. 28 30. The living ovum as seen when examined in normal 



salt-solution: a oxyphile body; b basiphile body; c yolk-nucleus. The 

 alveolar structure of the yolk-nucleus is noticeable. 



Plate 15. 



[Plathemis lydia.) 



Pig. 3l_38 are from cells stained with Plemming's triple stain. 

 Pig. 31—34. Yolk-nucleus breaking up in the cytoplasm. Lmm 

 reticulum of nucleus. Little or no chromatic reticulum. 



