APPENDIX. 863 



Glycogen in hifusoria. 



Glycogen, or animal starch, having been demonstrated by M. Claude Bernard 

 to be present as a product of nutrition in all higher animals, M. Certes * success- 

 fully experimented with a view to its detection in Infusoria. Under the reaction of 

 iodine it is revealed by the wine-red, purplish, or mahogany-red colour exhibited by 

 the component elements of the tissues that contain it. The action of the reagent 

 being regulated, it was found to be abundantly developed among the Infusoria, it 

 imparting to the various specimens examined the characteristic tints to the sarcode 

 element generally, but sparing the cuticle, vibratile cilia, nuclei, nucleoli, and con- 

 tractile vesicle ; and also, in the case of Vorticella, the contractile filament. 



Preservative Fluid for Infusoria. &c. 



Dr. T. F. Alien! finds the following solution suited to the permanent preservation 

 of Infusoria : — 



Wood vinegar, sp.gr. i"04 100 parts. 



Salicylic acid I part. 



The above compound he calls salicylic vinegar; to this add for salicylic 

 vinegar i part, glycerine 10 parts, and water 40 parts. 



* ' Comptes Rendus,' xc, p. 77, 1880. 



t 'American Monthly Microscopical Journal,' p. 98, 1881 ; also 'Journ. Roy. Mic. Soc.,' 

 October 1881. 



