Chakrison] viscous FERMENTATION OF MILK AND BEER 83 



Peptone solution 1 per cent -|- lactose 4 per cent. — The growth 

 in this meclmm was similar to that in Dunham's solution, but more 

 abundant. The surface la)^er became turbid and slimy, and later, the 

 whole medium, more or less. In 3 days a copious, viscid sediment 

 formed. No gas formed and the reaction remained unchanged. 



Peptone solution — 1 per cent, with the addition of the following 

 sugars : dextrose, 4 per cent ; saccharose, 4 per cent ; maltose, 4 per cent ; 

 glycerine, 4 per cent. Cultures in these media behaved exactly like 

 those in peptone-lactose solution. 



No indol formed in peptone solution. All representatives of the 

 group were strictly gerobic. 



They grew best at 25^ C, jvell at 15° to 20° C, and at 37.5'' C. 



The development was as good in neutral as in slightly alkaline 

 media. The organisms grew in media with % to 1 per cent normal 

 hydrochloric acid or 1 to 3 per cent nonnal sodium hydrate. 



Young and old cultures were killed by heating for 10 minutes at 

 60° C. 



The bacilli in agar and milk cultures remained alive for from 10 

 to 12 months. 



Coverglass preparations kept in broad daylight remained alive from 

 12 to 14 days. 



Group II. — Variety No. 6. 



Morphology. — Very motile, peritrichous bacillus, which usually 

 occurred singly, occasionally in short chains of a few cells. Irregular- 

 ities in size and shape were more pronounced than in representatives 

 of Group Î. The bacilli were thinner and longer than those of Group I. 



Sn'ze. — The bacilli were .5 to 3 ju long, and .25 to .75 ju wide ; 

 they did not take Gram's stain. 



Gelatine cultures. — In 24 hours colonies were round, slightly grey, 

 or water-like, clear and shiny and of pronounced viscosity. They grew 

 larger and the edge became irregular and lobulate, the surface remained 

 flnt and shiny. The colony gi-adually became umbonate and assumed 

 a citron-yellow colour, and the raised portion grew outward as far as 

 the edge of the colony. The largest colonies were o to 7 m.m. in dia- 

 meter. When touched with a platinum needle, the colony could be 

 drawn out to a thread, until the whole of it left the gelatine surface 

 and contracted to a drop on the needle. All colonies gradually sank 

 into the gelatine, that is to say, there was partial liquefaction. 



Microscopical. — In young cultures the centre part was slightly 

 brown and granular. The edge was dark brown or even black, between 

 the centre and edge there was a thin, light brown or greyish, clear zone. 



