86 ROYAL SOCIETY OF CANADA 



Gelatine streak cultures. — A shiny, grey, even growth formed along, 

 the line of inoculation, which spread very little from the line of inocu- 

 lation and soon became raised. 



Agar plate and streak cultures were similar to the gelatine cultures. 

 The growth of No. 7 on the sloped agar gradually flowed down into' 

 the condensation water, which later became a grey, mucous-like viscous 

 mass. 



Potato. — On this medium there was good growth which spread but 

 little. The growth was thick, even, very shiny, and grey ; later it became 

 slightly yellow and very viscid. 



Mill-. — The milk was apparently unchanged for 24 hours, but 

 viscous or sour. Slight shaldng or introducing a hot platinum needle 

 caused a considerable development of gas bubbles. The coagulum con- 

 tracted and pressed out a grey serum. The coagulum was viscous and 

 often full of large gas bubbles. 



Beef and whey bouillon. — In 24 hours the whole medium was very 

 turbid and viscid. On the surface a thin, grey pellicle and a thick,, 

 slimy ring formed. The reaction was slightly sour and small gas bub- 

 bles developed in the whey tube. The turbidity gradually increased, 

 the pellicle disappeared, and a copious grey sediment formed at the- 

 bottom of the tube. The acid reaction of the beef bouillon disappeared 

 in a few days and became neutral. The whey bouillon became and 

 remained strongly acid. Both cultures developed a disagreeable sweet- 

 ish smell. 



Peptone solution. — In one per cent peptone solution, with or without 

 salt, very good growth appeared in 24 hours, the liquid became very 

 turbid. The reaction did not alter. A sweetish disagreeable odour 

 developed and the whole medium was viscous. 



In peptone solution 1 per cent with the addition of lactose, 4 per 

 cent; glucose, 4 per cent; saccharose, 4 per cent; maltose, 4 per cent; 

 and glycerine, 4 per cent, there was fairly good growth, considerable 

 turbidity, and the formation of copious sediment in 18 to 24 hours. 

 In 3 to 5 days all media were viscous. Sliminess was partially pre- 

 vented by the production of acid which occurred in from 1 to 2 days, 

 when the viscosity gradually disappeared. 



There was much indol formed in cultures in Dunliam's medium.. 

 The bacilli grew well with or without oxygen, and cultures of both- 

 varieties were quite viscid. 



They grew well at 25° C. to 37° C, but better at the body tem- 

 perature. 



The best growth occurred in slightly alkaline (1 to 3 per cent 

 normal sodium hydrate) media, but good growth also took place in 

 slightly sour (i/o-l per cent Normal HCIi) media. 



