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ROYAL SOCIETY OF CANADA 



Medium. 



All Media 



Days. 



Sucrose, 

 Mannite and 

 Dextrose. 



Sucrose. 



30 to fiO 

 10 64 



8 to 10 

 to 24 



Galactose. 



Mannite. 



Lactose. 



Galactose. 



Dextrose. 



Glycerine. 



Maltose. 



No carbohy- 

 drate. 



Growth, Pigment, and Liquefaction. 



18 

 z4 



30 



64 

 18 

 24 



18 



18 

 24 



30 



1 



8 



24 

 30 



Growth in all raised, circular, could be drawn out 

 in slimy threads, white or else pigmented. Growth 

 along the line of inoculation slight and increasing- 

 little in 10 days or more. 



Liquefaction began in 8 days in mannite gelatin; 

 in 10 days in the others. 



It was crateriform or napiform, slowly becoming 

 stratiform or funnel form. 



In 30 days the maltose gelatin was liquefied to 

 the bottom. 



In 60 to 64 days all were liquefied to the bottom. 

 The liquefied gelatin was turbid, dark or inky above, 

 amber to rust colored below. There was much white 

 ropy sediment. 



In 24 hours there was much dark violet color 

 diffusing in the gelatin from the point of puncture, 

 most with sucrose. None in the other media in 24 

 hours. 



Growth 7 to S m.in. across, circular with wavy 

 outline, raised, wet-shining, and slimy. The growth 

 was bright blue toward the centre, light blue toward 

 the margins; the medium dark violet (gentian), 

 violet, and lilac to a depth of 15 m.m. 



All the gelatin was of a rich violet color. 



Liquefaction napiform. Surface growth a ragged, 

 lobulate, lead colored colony. The unliquefied 

 gelatin transparent, wine colored above and violet 

 at the bottom. 



Liquefaction funnel form, reaching half way to the 

 bottom, color lilac. 



Liquefied to the bottom, with an inky layer above. 



Blue and some violet in mediuin and in the growth. 



Liquefaction stratiform, the liquefied gelatin turbid, 

 with an upper darker layer, amber colored below, as 

 was also the unliquefied gelatin. 



Liquefaction crateriform with a light blue floating 

 colony; unliquefied gelatin bright blue and violet. 



Some violet pigment. 



Liquefaction stratiform, a smoky layer near the 

 surface; unliquefied gelatin amber. 



Liquefied to the bottom, amber colored, copious 

 sediment tough and ropy. 



Growth, no pigment. 



Surface growth 4 m.m. across, raised, moist- 

 shining, white. Growth along the line of puncture 

 slight, filiform. No pigment. 



Liquefaction stratiform, medium, a light yellow- 

 green color throughout. 



Stratiform, a slight darkening in color. 



