{HARRISON à barlow] SLIME- PRODUCING ORGANISM 



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Potash Gelatin with Carbohydrates. — 50 grams of gelatin were 

 soaked in 500 c. c. of decinormal KOH for three hours. The gelatin 

 was then washed in running tap water for 9 hours, when the washed 

 gelatin weighed 690 grams. After dissolving in steam the solution 

 was found to be alkaline — 3°. This medium is hereafter referred to 

 as K and served as a base for the following media: 



Medium K -|- sucrose 2^, referred to as KS. 



Medium K + 1 peptone and |^ salt, was steamed and filtered. 

 The filtrate was alkaline — 1°. This medium is referred to as KP, and 

 to 50 c. c. portions of it various carbohydrates were added, as follows: 



Sucrose 2^ referred to as K I* S. 



Lactose 2^ referred to as K P L. 



Dextrin 2((, referred to as K P Dn. 



Dextrose 2^ referred to as K P D. 



Glycerine 2^ referred to as K P G. 



Galactose 2^ referred to as K P Ga. 



All the above media were tubed and sterilized in flo\A'ing steam on 

 three successive days. 



Potash GeJaiin with Carhohydrates. — Stich cultures at 18° — 

 22° C. 



